NNT-1 Antibody
| Code | Size | Price |
|---|
| PSI-38-130-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
NNT-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
CLC, NR6, BSF3, NNT1, BSF-3, CISS2, NNT-1, CLC, Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3
Application Note:
ELISA:
Indirect:
To detect hNNT-1 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1.
Sandwich
To detect hNNT-1by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human NNT-1 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1.
Western Blot:
To detect hNNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
Indirect:
To detect hNNT-1 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1.
Sandwich
To detect hNNT-1by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human NNT-1 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1.
Western Blot:
To detect hNNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
Background:
NNT1 belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. IL6 family members share similarity in gene structure and have a 4 helix bundle in their protein structure.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNNT-1/BCSF-3. Human NNT-1/BCSF-3 specific antibody was purified by affinity chromatography employing immobilized hNNT-1/BCSF-3 matrix.
NCBI Gene ID #:
23529
NCBI Official Name:
cardiotrophin-like cytokine factor 1
NCBI Official Symbol:
CLCF1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q9UBD9
Protein GI Number:
56404673
Research Area:
Chemokines & Cytokines,Immunology,Antibody Pairs
Swissprot #:
Q9UBD9
User NOte:
Centrifuge vial prior to opening.