SAR1 Antibody

ProSci
Product Code: PSI-46-730
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-730-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-730 (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-730 (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
SAR1A, SAR1 gene homolog A (S. cerevisiae), SAR1, SARA1, Sara, masra2, OTTHUMP00000060603, SAR1a gene homolog 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 23 kDa band observed in lysates of NIH3T3 (calculated MW of 22.4 kDa according to Human NP_064535.1 and to Mouse NP_033146.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin and Small Intestine. Recommended concentration: 3.75ug/ml.
Background References:
  • Bi X, Mancias JD, Goldberg J. Insights into COPII coat nucleation from the structure of Sec23.Sar1 complexed with the active fragment of Sec31. with the active fragment of Sec31. Dev Cell. 2007 Nov;13(5):635-45.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-HSRLVESKVELNA
NCBI Gene ID #:
56681
NCBI Official Name:
SAR1 homolog A (S. cerevisiae)
NCBI Official Symbol:
SAR1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_064535.1
Protein GI Number:
9910542
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
Q9NR31