SPAG16 Antibody
| Code | Size | Price |
|---|
| PSI-42-305-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
SPAG16, sperm associated antigen 16, DKFZp666P1710, FLJ22724, FLJ37717, MGC87036, PF20, WDR29, WD repeat domain 29, sperm-associated WD repeat protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 70.8kDa according to NP_078808.3 and of 20.6kDa according to NP_001020607.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:This antibody was successfully used in Human and Murine frozen Brain sections (PMID: 25086173).
Background References:
- Zhang Z, Zariwala MA, Mahadevan MM, Caballero-Campo P, Shen X, Escudier E, Duriez B, Bridoux AM, Leigh M, Gerton GL, Kennedy M, Amselem S, Knowles MR, Strauss JF, A heterozygous mutation disrupting the SPAG16 gene results in biochemical instability of central apparatus components of the human sperm axoneme. Biology of reproduction 2007 Nov 77 (5): 864-71.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KGVTELRTVGNVPD
NCBI Gene ID #:
79582
NCBI Official Name:
sperm associated antigen 16
NCBI Official Symbol:
SPAG16
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_078808.3, NP_001020607.1
Protein GI Number:
70909324
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1).
Swissprot #:
Q8N0X2