SPARTIN Antibody

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ProSci
Product Code: PSI-42-286
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-286-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-286 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-286 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
SPG20, spastic paraplegia 20 (Troyer syndrome), KIAA0610, SPARTIN, TAHCCP1, OTTHUMP00000018254, spastic paraplegia 20, spastic paraplegia 20, spartin (Troyer syndrome), trans-activated by hepatitis C virus core protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in lysates of cell line HeLa (calculated MW of 72.8kDa according to NP_055902.1). Recommended concentration: 1-3ug/ml.Immunofluorescence: HeLa show dimished signals after knock down of expresssion by introduction of siRNA.
Background References:
  • Lu J, Rashid F, Byrne PC. The hereditary spastic paraplegia protein spartin localises to mitochondria J Neurochem. 2006 Sep;98(6):1908-19.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-EASGTDVKQLDQGNK
NCBI Gene ID #:
23111
NCBI Official Name:
spastic paraplegia 20 (Troyer syndrome)
NCBI Official Symbol:
SPG20
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055902.1
Protein GI Number:
21703346
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Swissprot #:
Q8N0X7