SPHK1 Antibody

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ProSci
Product Code: PSI-46-726
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-726-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-726 (2ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-726 (2ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
SPHK1, sphingosine kinase 1, SPHK, SK1, SKase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3ug/ml.Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected.
Background References:
  • Kase H, Hattori Y, Jojima T, Okayasu T, Tomizawa A, Suzuki K, Banba N, Monden T, Satoh H, Akimoto K, Kasai K. Globular adiponectin induces adhesion molecule expression through the sphingosine kinase pathway in vascular endothelial cells. Life Sci. 2007 Aug 23;81(11):939-43. Epub 2007 Aug 17.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DVDLESEKYRRLGE
NCBI Gene ID #:
8877
NCBI Official Name:
sphingosine kinase 1
NCBI Official Symbol:
SPHK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_068807.2, NP_892010.2, NP_001136073.1
Protein GI Number:
21361088
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all three reported isoforms (NP_068807.2; NP_892010.2; NP_001136073.1). Reported variants represent identical protein (NP_001136073.1; NP_001136074.1).
Swissprot #:
Q9NYA1