UNC45B Antibody

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ProSci
Product Code: PSI-42-281
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-281-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-281 (0.3ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-281 (0.3ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
unc-45 homolog B (C. elegans), CMYA4, FLJ38610, MGC119540, MGC119541, UNC45, cardiomyopathy associated 4, striated muscle UNC45
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 90kDa band observed in Human Skeletal Muscle lysates (calculated MW of 104kDa according to NP_775259.1). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Landsverk ML, Li S, Hutagalung AH, Najafov A, Hoppe T, Barral JM, Epstein HF. The UNC-45 chaperone mediates sarcomere assembly through myosin degradation in Caenorhabditis elegans. J Cell Biol. 2007 Apr 23;177(2):205-10. Epub 2007 Apr 16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-RSDKLRQKIFKER
NCBI Gene ID #:
146862
NCBI Official Name:
unc-45 homolog B (C. elegans)
NCBI Official Symbol:
UNC45B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_775259.1, NP_001028748.1
Protein GI Number:
27477061
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_775259.1and NP_001028748.1).
Swissprot #:
Q8IWX7