Vinculin Antibody

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ProSci
Product Code: PSI-7807
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7807-0.02mg0.02mg£150.00
Quantity:
PSI-7807-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Vinculin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Vinculin 7489 (2 μg/mL), Vinculin 7807 (1 μg/mL), Vinculin 7809 (1 μg/mL), and beta-actin 3779 (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Vinculin 7807 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in PC3 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Vinculin 7807 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Western Blot Validation in Human Liver Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Vinculin 7807 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 Western Blot Validation in Rat Liver Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Vinculin 7807 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 6 Immunocytochemistry Validation of Vinculin in Jurkat Cells</strong><br> Immunocytochemical analysis of Jurkat cells using anti-Vinculin antibody (7807) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 7 Immunofluorescence Validation of Vinculin in Jurkat Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Vinculin with 7807 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Vinculin 7489 (2 μg/mL), Vinculin 7807 (1 μg/mL), Vinculin 7809 (1 μg/mL), and beta-actin 3779 (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Vinculin 7807 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in PC3 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Vinculin 7807 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Human Liver Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Vinculin 7807 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Western Blot Validation in Rat Liver Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Vinculin 7807 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 6 Immunocytochemistry Validation of Vinculin in Jurkat Cells</strong><br> Immunocytochemical analysis of Jurkat cells using anti-Vinculin antibody (7807) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunofluorescence Validation of Vinculin in Jurkat Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Vinculin with 7807 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

Documents

Further Information

Additional Names:
CMD1W, CMH15, Metavinculin, MVCL
Application Note:
WB: 0.5-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
Background References:
  • Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525.
  • Gilmore et al. J. Cell Sci. 1992; 103:719-31.
  • Deakin et al. PLoS One 2012; 7:e37990.
  • Goldmann et al. Cell Biol. Int. 2013; Feb 1.
Buffer:
Vinculin antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-Vinculin antibody (7807) was raised against a peptide corresponding to 23 amino acids near the amino terminus of human Vinculin.
ISOFORMS:
Human Vinculin has 3 isoforms, including isoform 2 (1134aa, 124kD), isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD). Mouse Vinculin has one isoform (1066aa, 117kD) and Rat Vinculin also has one isoform (1066aa, 117kD). 7807 can detect human, mouse and rat.
NCBI Gene ID #:
7414
NCBI Official Name:
vinculin
NCBI Official Symbol:
VCL
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 124kD

Observed: 124kD
Protein Accession #:
NP_003364
Protein GI Number:
4507877
Purification:
Vinculin antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist.
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar Vinculin expression profile in human, mouse and rat cell lines detected by three independent anti-Vinculin antibodies that recognize different epitopes, 7489 against C-terminus domain, 7807 against N-terminus domain and 7809 against central domain.  Vinculin proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies. 

References

  1. Wang et al. AMPK is involved in the differential neonatal performance of chicks hatching at different time. Gen Comp Endocrinol. 2016;228:53-59. PMID: 26873631