Mouse anti Cardiotin
| Code | Size | Price |
|---|
| MUB0307P | 0.1 ml | £326.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgM
Antibody Clonality: Monoclonal
Antibody Clone: R2G
Regulatory Status: RUO
Target Species:
- Amphibian
- Canine (dog)
- Caprine (goat)
- Feline (cat)
- Fish
- Hamster
- Human
- Monkey
- Mouse
- Rabbit
- Rat
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store at 4°C or in small aliquots at -20°C.
Documents
Further Information
Applications Description:
R2G is useful for immunohistochemistry on frozen and paraffin-embedded tissue and immunoblotting. In immunoblotting assays R2G reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. Recommended range is 1:25 ? 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent. Optimal antibody dilution for immunoblotting applications should be determined by titration.
Background:
Cardiotin is a high molecular weight protein complex (300 kDa) loCated in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuRation. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to Human skeletal muscle ?-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Concentration:
1 mg/ml
Field of Interest:
Cardiovascular
Formulation:
Each vial contains 100 ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Product:
Each vial contains 100 ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Purification Method:
Ammonium sulphate precipitation
Source:
R2G is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells, with spleen cells from a mouse immunized with a total protein extract of chicken gizzard.
Specificity:
R2G reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle.
UniProt:
multiple
References
1. Schaart, G., van der Ven, P. F., and Ramaekers, F. C. (1993). Characterization of cardiotin, a structural component in the myocard, Eur J Cell Biol 62, 34-48.
2. Schaart, G., Moens, L., Endert, J. M., and Ramaekers, F. C. (1997). Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein, FEBS Lett 403, 168-72.
3. Ausma, J., Wijffels, M., van Eys, G., Koide, M., Ramaekers, F., Allessie, M., and Borgers, M. (1997). Dedifferentiation of atrial cardiomyocytes as a result of chronic atrial fibrillation, Am J Pathol 151, 985-97.
4. Dispersyn, G. D., Geuens, E., Ver Donck, L., Ramaekers, F. C., and Borgers, M. (2001). Adult Rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts, Cardiovasc Res 51, 230-40.
5. Ausma, J., Litjens, N., Lenders, M-H., Duimel, H., Mast, F., Wouters, L., Ramaekers, F., Allessie, M., and Borgers, M. (2001). Time course of atrial fibrillation-induced cellular structural remodeling in atria of the goat, J Mol Cell Cardiol 33, 2083-94.
6. Dispersyn, G. D., Mesotten, L., Meuris, B., Maes, A., Mortelmans, L., Flameng, W., Ramaekers, F. C., and Borgers, M. (2002). Dissociation of cardiomyocyte apoptosis and dedifferentiation in infarct border zones, Eur Heart J in press.
7. Ausma, J., van der Velden, H. M., Lenders, M. H., van Ankeren, E. P., Jongsma, H. J., Ramaekers, F. C., Borgers, M., and Allessie, M. A. (2003). Reverse structural and gap-junctional remodeling after prolonged atrial fibrillation in the goat. Circulation 107, 2051-2058.
8. RR Pochampally, BT Neville, EJ Schwarz, MM Li, DJ Prockop (2004). Rat adult stem cells (marrow stromal cells) engraft and differentiate in chick embryos without evidence of cell fusion, Proc Natl Acad Sci USA 101, 9282-85
2. Schaart, G., Moens, L., Endert, J. M., and Ramaekers, F. C. (1997). Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein, FEBS Lett 403, 168-72.
3. Ausma, J., Wijffels, M., van Eys, G., Koide, M., Ramaekers, F., Allessie, M., and Borgers, M. (1997). Dedifferentiation of atrial cardiomyocytes as a result of chronic atrial fibrillation, Am J Pathol 151, 985-97.
4. Dispersyn, G. D., Geuens, E., Ver Donck, L., Ramaekers, F. C., and Borgers, M. (2001). Adult Rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts, Cardiovasc Res 51, 230-40.
5. Ausma, J., Litjens, N., Lenders, M-H., Duimel, H., Mast, F., Wouters, L., Ramaekers, F., Allessie, M., and Borgers, M. (2001). Time course of atrial fibrillation-induced cellular structural remodeling in atria of the goat, J Mol Cell Cardiol 33, 2083-94.
6. Dispersyn, G. D., Mesotten, L., Meuris, B., Maes, A., Mortelmans, L., Flameng, W., Ramaekers, F. C., and Borgers, M. (2002). Dissociation of cardiomyocyte apoptosis and dedifferentiation in infarct border zones, Eur Heart J in press.
7. Ausma, J., van der Velden, H. M., Lenders, M. H., van Ankeren, E. P., Jongsma, H. J., Ramaekers, F. C., Borgers, M., and Allessie, M. A. (2003). Reverse structural and gap-junctional remodeling after prolonged atrial fibrillation in the goat. Circulation 107, 2051-2058.
8. RR Pochampally, BT Neville, EJ Schwarz, MM Li, DJ Prockop (2004). Rat adult stem cells (marrow stromal cells) engraft and differentiate in chick embryos without evidence of cell fusion, Proc Natl Acad Sci USA 101, 9282-85