Rat anti Ep-CAM / CD326
| Code | Size | Price |
|---|
| MUB0509P | 0.1 mg | £326.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: G8.8
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Flow Cytometry
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Storage:
Store at 4°C or in small aliquots at -20°C.
Documents
Further Information
Applications Description:
G8.8 is suitable for immunoblotting, immunoprecipitation, immunocytochemistry and immuno-histochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:25 ? 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 ? 1:1000 for immunoblotting applications.
Background:
Ep-CAM is a 40 kD glycoprotein and can be detected at the basolateral membrane of the majority of epithelial tissues, where it is intriCately linked with the cadherin-Catenin complex and hence the fundamental WNT pathway responsible for intracellular signalling and polarity. This antigen functions as a homotypic calcium-independent cell adhesion molecule. Of particular interest, Ep-CAM appears to be overexpressed by the majority of Human epithelial carcinomas, including colorectal, breast, prostate, head and neck, and hepatic carcinomas. The antigen is being used as a target for immunotherapy of Human carcinomas. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial prolifeRation.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Field of Interest:
Cancer|Cell adhesion
Formulation:
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Product:
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Source:
G8.8 is a rat monoclonal IgG2a antibody derived by fusion of X63-Ag8.653 mouse myeloma cells with spleen lymphocytes from rats repeatedly immunized with glycoconjugates from BALB/c Mouse derived medullary thymic epithelial cells.
Specificity:
The G8.8 antibody reacts with CD326/Ep-CAM (Epithelial Cell Adhesion Molecule), also known as gp-40 in the Mouse. Ep-CAM is a 40-42-kDa cell-surface glycoprotein expressed on thymic epithelial cells, thymic dendritic cells, immature thymocytes, a small subset of peripheral T lymphocytes, intestinal epithelium, kidney-collecting tubule epithelium, Keratinocytes, Langerhans cells, as well as lymph node and splenic dendrititc cells.
UniProt:
Q99JW5
References
1. Farr, A., Nelson, A., Truex, J. And Hosier, S. (1991). Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium. The Journal of Histochemistry and Cytochemistry 39, 645-53.
2. Lee, M., Sharrow, S., Farr, A., Singer, A. and Udey M. (1994). Expression of the homotypic adhesion molecule E-cadherin by immature murine thymocytes and thymic epithelial cells. Journal of Immunology Mol 152, 5653-59.
3. Borkowski, T., Nelson, A., Farr, A. and Udey, M. (1996). Expression of gp40, the murine homologue of Human epithelial cell adhesion molecule (Ep-Cam), by murine dendritic cells. Eur. J. Immunol. 26, 110-14.
4. Nelson, A., Dunn, R., Peach, R., Aruffo, A. and Farr, A. (1996). The murine homolog of Human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells. Eur. J. Immunol. 26, 401-408.
5. Went, P., Vasei, M., Bubendorf, L., Terracciano, L., Tornillo, L., Riede, U., Kononen, J., Simon, R., Sauter, G. And Baeuerle, P.A. (2006). Frequent high-level expression of the immunotherapeutic target Ep-CAM in colon, stomach, prostate and lung cancers bladder. British Journal of Cancer 94, 128-135.
6. Maaser, K. and Borlak, J. (2008). A genom-wide expression analysis identifies a network of EpCAM-induced cel cycle regulators. British Journal of Cancer 99, 1635-43
2. Lee, M., Sharrow, S., Farr, A., Singer, A. and Udey M. (1994). Expression of the homotypic adhesion molecule E-cadherin by immature murine thymocytes and thymic epithelial cells. Journal of Immunology Mol 152, 5653-59.
3. Borkowski, T., Nelson, A., Farr, A. and Udey, M. (1996). Expression of gp40, the murine homologue of Human epithelial cell adhesion molecule (Ep-Cam), by murine dendritic cells. Eur. J. Immunol. 26, 110-14.
4. Nelson, A., Dunn, R., Peach, R., Aruffo, A. and Farr, A. (1996). The murine homolog of Human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells. Eur. J. Immunol. 26, 401-408.
5. Went, P., Vasei, M., Bubendorf, L., Terracciano, L., Tornillo, L., Riede, U., Kononen, J., Simon, R., Sauter, G. And Baeuerle, P.A. (2006). Frequent high-level expression of the immunotherapeutic target Ep-CAM in colon, stomach, prostate and lung cancers bladder. British Journal of Cancer 94, 128-135.
6. Maaser, K. and Borlak, J. (2008). A genom-wide expression analysis identifies a network of EpCAM-induced cel cycle regulators. British Journal of Cancer 99, 1635-43