Mouse anti ICAM-3

MA4 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 ? 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 ? 1:1000 for immunoblotting applications.

Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a type I transmembrane glycoprotein, which normally contain 2-9 immunoglobulin-like C2-type domains, and binds to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 has previously been implicated in apoptotic cell clearance, although its precise role in the clearance process is ill defined.

Immunology|Apoptosis

Each vial contains 100 ?l 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Each vial contains 100 ?l 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

MA4 is a mouse monoclonal IgG1/k antibody derived by fusion of mouse Ag8.653 cells with spleen cells from a BALB/c mouse immunized with an ICAM-3/HEK transfectant.

MA4 recognizes a linear epitope in domains 1 and 2 of ICAM-3. MA4 robustly inhibits apoptotic cell interaction with phagocytes

P32942