DDDDK-tag Antibody

ProSci
Product Code: PSI-7873
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7873-0.02mg0.02mg£150.00
Quantity:
PSI-7873-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
DDDDK-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of a DDDDK-tag-containing recombinant protein with DDDDK-tag antibody at (A) 0.125 and (B) 0.25 μg/ml.
2 / 2
Western blot analysis of 20ng of DDDDK-tagged recombinant GGP1 protein using 1 μg of DDDDK antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.

Western blot analysis of a DDDDK-tag-containing recombinant protein with DDDDK-tag antibody at (A) 0.125 and (B) 0.25 μg/ml.
Western blot analysis of 20ng of DDDDK-tagged recombinant GGP1 protein using 1 μg of DDDDK antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.

Further Information

Additional Names:
DDDDK-tag, DDDDK, DDDDK-tag, DDDDK, flag-tag, flag
Application Note:
DDDDK-tag antibody can be used for detection of DDDDK-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.
Background:
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. DDDDK-tag is one of the more popular epitope tags for expressed recombinant proteins.
Buffer:
DDDDK-tag antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DDDDK-tag antibody was raised against a synthetic peptide containing DYKDDDDK.
NCBI Official Name:
N/A
NCBI Official Symbol:
N/A
NCBI Organism:
N/A
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
N/A
Purification:
DDDDK-tag antibody is affinity chromatography purified via peptide column.
Research Area:
Other
SPECIFICITY:
DDDDK-tag antibody will detect amino- or carboxy-terminal DDDDK-tagged recombinant proteins.
User NOte:
Optimal dilutions for each application to be determined by the researcher.