GABARAPL2 Antibody
| Code | Size | Price |
|---|
| PSI-7895-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7895-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 6005768
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
GABARAPL2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
GABARAPL2 Antibody: ATG8, GEF2, ATG8C, GEF-2, GATE16, GATE-16, FLC3A, Gamma-aminobutyric acid receptor-associated protein-like 2, Ganglioside expression factor 2
Application Note:
GABARAPL2 antibody can be used for detection of GABARAPL2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Background:
Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system (1). It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC (2). GABARAPL2 (GABAA receptor-associated protein-like 2), also known as GATE16, was initially identified as a membrane transport modulator and is a mammalian ortholog to the autophagy protein ATG8 (3,4). It is thought that GABARAPL2 and other members of the ATG8 family act as scaffolds for assembly of the Unc-51 like kinase (ULK) complex in the formation of autophagosomes (5).
Background References:
- Cherubini E, Gaiarsa JL, and Ben-Ari Y. GABA: an excitatory transmitter in early postnatal life. Trends Neurosci.1991; 14:515-19.
- Dirkx R Jr, Thomas A, Li L, et al. Targeting of the 67 kDa isoform of glutamic acid decarboxylase to intracellular organelles is mediated by its interaction with the NH2-terminal region of the 65 kDa isoform of glutamic acid decarboxylase. J. Biol. Chem. 1995; 270:2241-6.
- Sagiv Y, Legesse-Miller A, Porat A, et al. GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28. EMBO J. 2000; 19:1494-504.
- Paz Y, Elazar Z, and Fass D. Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p. J. Biol. Chem. 2000; 275:25445-50.
Buffer:
GABARAPL2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
GABARAPL2 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human GABARAPL2.
The immunogen is located within the last 50 amino acids of GABARAPL2.
The immunogen is located within the last 50 amino acids of GABARAPL2.
NCBI Gene ID #:
11345
NCBI Official Name:
GABA(A) receptor-associated protein-like 2
NCBI Official Symbol:
GABARAPL2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 13 kDa
Observed: 13 kDa
Observed: 13 kDa
Protein Accession #:
NP_009216
Protein GI Number:
6005768
Purification:
GABARAPL2 antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
GABARAPL2 antibody is human, mouse and rat reactive. Multiple isoforms of GABARAPL2 are known to exist.
Swissprot #:
P60520
User NOte:
Optimal dilutions for each application to be determined by the researcher.