ATG4C Antibody
| Code | Size | Price |
|---|
| PSI-7907-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7907-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 30410846
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
ATG4C antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
ATG4C Antibody: APG4C, AUTL1, AUTL3, APG4-C, APG4C, Cysteine protease ATG4C, AUT-like 3 cysteine endopeptidase
Application Note:
ATG4C antibody can be used for detection of ATG4C by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4C, also known as autophagin-3, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). Recent studies have shown that ATG4C and Beclin-1 are regulated by the microRNA miR-376b; overexpression of miR-376b led to decreased mRNA and protein levels, thereby blocking starvation and TOR inhibition-related autophagy (4).
Background References:
- Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
- Marino G, Uria JA, Puente XS, et al. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy. J. Biol. Chem. 2003; 278:3671-8.
- Korkmaz G, le Sage C, Tekirdag KA, et al. miR-376b controls starvation and TOR inhibition-related autophagy by targeting ATG4C and BECN1. Autophagy 2012; 8:165-76.
Buffer:
ATG4C antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ATG4C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4C.
The immunogen is located within the last 50 amino acids of ATG4C.
The immunogen is located within the last 50 amino acids of ATG4C.
NCBI Gene ID #:
84938
NCBI Official Name:
autophagy related 4C, cysteine peptidase
NCBI Official Symbol:
ATG4C
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 50 kDa
Observed: 57 kDa
Observed: 57 kDa
Protein Accession #:
NP_835739
Protein GI Number:
30410846
Purification:
ATG4C antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
ATG4C antibody is human, mouse and rat reactive. ATG4C is predicted to not cross-react with other ATG4 proteins.
Swissprot #:
Q96DT6
User NOte:
Optimal dilutions for each application to be determined by the researcher.