Rubicon Antibody
| Code | Size | Price |
|---|
| PSI-7943-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7943-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 530375960
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Rubicon antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
Run domain Beclin-1 interacting and cysteine-rich containing protein, KIAA0226
Application Note:
Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).
Background References:
- Matsunaga K, Saitoh T, Tabata K, et al. Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages. Nat. Cell Biol. 2009; 11:385-96.
- Yang CS, Lee JS, Rodgers M, et al. Autophagy protein Rubicon mediates phagocytic NAPDH oxidase activation in response to microbial infection of TLR stimulation. Cell Host and Microbe 2012; 11:264-76.
Buffer:
Rubicon antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.
The immunogen is located within amino acids 240 - 290 of Rubicon.
The immunogen is located within amino acids 240 - 290 of Rubicon.
NCBI Gene ID #:
9711
NCBI Official Name:
KIAA0226
NCBI Official Symbol:
KIAA0226
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 90, 101, 109 kDa
Observed: 91, 99 kDa
Observed: 91, 99 kDa
Protein Accession #:
XP_005269431
Protein GI Number:
530375960
Purification:
Rubicon antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.
Swissprot #:
Q92622
User NOte:
Optimal dilutions for each application to be determined by the researcher.