UVRAG Antibody
| Code | Size | Price |
|---|
| PSI-7947-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7947-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 21687212
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
UVRAG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
UVRAG Antibody: p63, DHTX, VPS38, UV radiation resistance-associated gene protein, p63
Application Note:
UVRAG antibody can be used for detection of UVRAG by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The class III type phosphoinositide 3-kinase (PI3KC3) / Vps34 regulates vacuolar trafficking as well as autophagy (1). UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (2). UVRAG is highly expressed in brain, lung, kidney and liver and contains one C2 domain, which is involved in calcium-dependent phospholipid binding. Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (3). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (4,5).
Background References:
- Stack JH, DeWald DB, Takegawa K, et al. Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. J. Cell Biol. 1995; 129:321-34.
- Liang C, Feng P, Ku B, et al. Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG. Nat. Cell Biol. 2006; 8:688-99.
- Liang C, Lee JS, Inn KS, et al. Beclin1-binding UVRAG targets the class C Vps complex to coordinate autophagosome maturation and endocytic trafficking. Nat. Cell Biol. 2008; 10:776-87.
- Ionov Y, Nowak N, Perucho M, et al. Manipulation of nonsense mediated decay identifies gene mutations in colon cancer Cells with microsatellite instability. Oncogene 2004; 23:639-45.
Buffer:
UVRAG antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
UVRAG antibody was raised against an 18 amino acid peptide near the carboxy terminus of human UVRAG.
The immunogen is located within amino acids 490 - 540 of UVRAG.
The immunogen is located within amino acids 490 - 540 of UVRAG.
NCBI Gene ID #:
7405
NCBI Official Name:
UV radiation resistance associated
NCBI Official Symbol:
UVRAG
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 77 kDa
Observed: 75 kDa
Observed: 75 kDa
Protein Accession #:
NP_003360
Protein GI Number:
21687212
Purification:
UVRAG antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
UVRAG antibody is human and mouse reactive.
Swissprot #:
Q9P2Y5
User NOte:
Optimal dilutions for each application to be determined by the researcher.