MAP1LC3 Antibody
| Code | Size | Price |
|---|
| PSI-7951-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7951-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 14210522
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
MAP1LC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
MAP1LC3 Antibody: LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3Autophagy-related protein LC3 A
Application Note:
MAP1LC3 antibody can be used for detection of MAP1LC3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3 is a subfamily of three related proteins belonging to the MAP1 LC3 family and it includes MAP1LC3A, MAP1LC3B, and MAP1LC3C (2). MAP1LC3 is the mammalian homolog of yeast ATG8 and is essential for autophagy and associated with the autophagosome membranes after processing (3). The three isoforms exhibit distinct expression patterns and both MAP1LC3A and MAP1LC3B but not MAP1LC3B, are post-translationally modified, suggesting the three isoforms may have different physiological functions (4).
Background References:
- Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81.
- Fink JK, Jones SM, Esposito C, et al. Human microtubule-associated protein 1A (MAP1A) gene: genomic organization, cDNA sequence, and developmental and tissue-specific expression. Genomics 1996; 35:577-85.
- Kabeya Y, Mizushima N, Ueno T, et al. LC3, a mammalian homolog of yeast Apg8p, is localized in autophagosome membrane after processing. EMBO J. 2000; 19:5720-8.
- He H, Dang Y, Dai F, et al. Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. J. Biol. Chem. 2003; 278:29278-87.
Buffer:
MAP1LC3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
MAP1LC3 antibody was raised against a 12 amino acid peptide near the center of human MAP1LC3A.
The immunogen is located within amino acids 20 - 70 of MAP1LC3.
The immunogen is located within amino acids 20 - 70 of MAP1LC3.
NCBI Gene ID #:
84557
NCBI Official Name:
microtubule-associated protein 1 light chain 3 alpha
NCBI Official Symbol:
MAP1LC3A
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 13 kDa
Observed: 18 kDa
Observed: 18 kDa
Protein Accession #:
NP_115903
Protein GI Number:
14210522
Purification:
MAP1LC3 antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
MAP1LC3 antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3 are known to exist. MAP1LC3 antibody is predicted to detect MAP1LC3A, MAP1LC3B, and MAP1LC3C.
Swissprot #:
Q9H492
User NOte:
Optimal dilutions for each application to be determined by the researcher.