PINK1 Antibody
| Code | Size | Price |
|---|
| PSI-7859-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7859-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 14165272
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
PINK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
PINK1 Antibody: BRPK, PARK6BRPK
Application Note:
PINK1 antibody can be used for detection of PINK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
The PTEN-induced putative kinase 1 (PINK1) is a serine/threonine protein kinase that localizes to mitochondria and is thought to protect cells from stress-induced mitochondrial dysfunction (reviewed in 1). PINK1 recruits the E3 ubiquitin ligase Parkin to mitochondria to initiate mitophagy, an autophagic process that clears damaged mitochondria within a cell (2). PINK1 is cleaved by the mitochondrial protease PARL (3). Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease (4).
Background References:
- Matsuda S, Kitagishi Y, and Kobayashi M. Function and characteristics of PINK1 in mitochondria. Oxid. Med. Cell. Longev. 2013;601587.
- Corti O, Lesage S, and Brice A. What genetics tells us about the causes and mechanism of Parkinson's disease. Physiol. Rev. 2011; 91:1161-218.
- Deas E, Plun-Favreau H, and Gandhi S, et al. PINK1 cleavage at position A103 by the mitochondrial protease PARL. Hum. Mol. Genet. 2011; 20:867-79.
- Valente EM, Abou-Sleiman PM, Caputo V, et al. Hereditary early-onset Parkinson's disease caused by mutations in PINK1. Science 2004; 304:1158-60.
Buffer:
PINK1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PINK1 antibody was raised against a 16 amino acid peptide near the amino terminus of human PINK1.
The immunogen is located within amino acids 120 - 170 of PINK1.
The immunogen is located within amino acids 120 - 170 of PINK1.
NCBI Gene ID #:
65018
NCBI Official Name:
PTEN induced putative kinase 1
NCBI Official Symbol:
PINK1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 55 kDa
Observed: 53 kDa
Observed: 53 kDa
Protein Accession #:
NP_115785
Protein GI Number:
14165272
Purification:
PINK1 antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy,Neuroscience
SPECIFICITY:
PINK1 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will only detect the longer isoform. PINK1 antibody will detect the cleaved and uncleaved form of PINK1.
Swissprot #:
Q9BXM7
User NOte:
Optimal dilutions for each application to be determined by the researcher.