TXNIP Antibody
| Code | Size | Price |
|---|
| PSI-7941-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7941-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 171184421
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
TXNIP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
Thioredoxin-interacting protein, BMP and activin membrane-bound inhibitor homolog, non-metastatic gene A protein, NMA
Application Note:
TXNIP body can be used for detection of TXNIP by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Thioredoxin-interacting protein (TXNIP) belongs to the arrestin family and plays a critical role in the antioxidant defense mechanisms of hematopoietic cells by activating the p53 pathway during oxidative stress (1,2). It functions as a transcriptional repressor and acts as an oxidative stress mediator by inhibiting thioredoxin activity (2). TXNIP expression is reduced in many types of tumors, and TXNIP overexpression inhibits tumor growth by blocking cell-cycle progression (3). It has recently reported that TXNIP deficiency correlates with a high incidence of hepatocellular carcinoma (HCC) (4). TXNIP and p53 interactions could potentially be a therapeutic target for oxidative stress-related diseases such as hematopoietic malignancies and metabolic diseases (5).
Background References:
- Chen KS and DeLuca HF. Isolation and characterization of a novel cDNA from HL-60 cells treated with 1, 25-dihydroxyvitamin D-3. Biochim. Biophys. Acta. 1994; 1219:26-32.
- Jeong M, Piao ZH, Kim MS, et al. Thioredoxin-interacting protein regulates hematopoietic stem cell quiescence and mobilization under stress conditions. J. Immunol. 2009; 183:2495?505.
- Han SH, Jeon JH, Ju HR, et al. VDUP1 upregulated by TGF-beta1 and 1,25-dihydorxyvitamin D3 inhibits tumor cell growth by blocking cell-cycle progression. Oncogene 2003; 22:4035?46.
- Kwon HJ, Won YS, Yoon YD, et al. Vitamin D3 up-regulated protein 1 deficiency accelerates liver regeneration after partial hepatectomy in mice. J. Hepatol. 2011; 54:1168?76.
Buffer:
TXNIP antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (94%)
Immunogen:
TXNIP antibody was raised against a 16 amino acid peptide near the center of human TXNIP.
The immunogen is located within amino acids 110 - 160 of TXNIP.
The immunogen is located within amino acids 110 - 160 of TXNIP.
NCBI Gene ID #:
10628
NCBI Official Name:
thioredoxin interacting protein
NCBI Official Symbol:
TXNIP
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 43 kDa
Observed: 39 kDa
Observed: 39 kDa
Protein Accession #:
NP_006463
Protein GI Number:
171184421
Purification:
TXNIP antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
SPECIFICITY:
TXNIP antibody is human, mouse and rat reactive.
Swissprot #:
Q9H3M7
User NOte:
Optimal dilutions for each application to be determined by the researcher.