LIN54 Antibody

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ProSci
Product Code: PSI-7933
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7933-0.02mg0.02mg£150.00
Quantity:
PSI-7933-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 169234719
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
LIN54 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of LIN54 in HeLa cell lysate with LIN54 antibody at 1 μg/ml.
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Immunocytochemistry of LIN54 in HeLa cells with LIN54 at 2.5 μg/mL.
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Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.
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Immunocytochemistry of LIN54 in HeLa cells with LIN54 antibody at 10 μg/ml.
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Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.<br><br>Red: LIN54 Antibody (7933) <br> Blue: DAPI staining
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Western blot analysis of LIN54 in HeLa cell lysate with LIN54 antibody at 1 μg/ml.
Immunocytochemistry of LIN54 in HeLa cells with LIN54 at 2.5 μg/mL.
Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.
Immunocytochemistry of LIN54 in HeLa cells with LIN54 antibody at 10 μg/ml.
Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.<br><br>Red: LIN54 Antibody (7933) <br> Blue: DAPI staining

Documents

Further Information

Additional Names:
LIN-54, JC8.6, CXCDC1, MIP120
Application Note:
LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes. This complex functions as a transcriptional repressor by binding to promoters of E2F target genes that regulate the G1/S transition (1). LIN54 is required for cell cycle progression and can bind to the cdc2 promoter in a sequence-specific manner (2).
Background References:
  • Schmit F, Cremer S, and Gaubatz S. LIN54 is an essential core subunit of the DREAM/LINC complex that binds to the cdc2 promotoer in a sequence-specific manner. FEBS J. 2009; 276:5703-16.
  • Matsuo T, Kuramoto H, Kumazaki T, et al. LIN54 harboring a mutation in CHC domain is localized to the cytoplasm and inhibits cell cycle progression. Cell Cycle 2012; 11:3227-36.
Buffer:
LIN54 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54.

The immunogen is located within the last 50 amino acids of LIN54.
NCBI Gene ID #:
132660
NCBI Official Name:
lin-54 homolog (C. elegans)
NCBI Official Symbol:
LIN54
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 88 kDa

Observed: 82 kDa
Protein Accession #:
NP_919258
Protein GI Number:
169234719
Purification:
LIN54 antibody is affinity chromatography purified via peptide column.
Research Area:
Cell Cycle
SPECIFICITY:
LIN54 antibody is human, mouse, and rat reactive. Multiple isoforms of LIN54 are known to exist.
Swissprot #:
Q6MZP7
User NOte:
Optimal dilutions for each application to be determined by the researcher.