HDAC1 Antibody

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ProSci
Product Code: PSI-7897
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7897-0.02mg0.02mg£150.00
Quantity:
PSI-7897-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 13128860
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
HDAC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of HDAC1 in human brain tissue lysate with HDAC1 antibody at 1μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Western blot analysis of HDAC1 in human brain tissue lysate with HDAC1 antibody at 1μg/ml in (A) the absence and (B) the presence of blocking peptide.

Documents

Further Information

Additional Names:
HDAC1 Antibody: HD1, RPD3, GON-10, RPD3L1, Histone deacetylase 1, HD1
Application Note:
HDAC1 antibody can be used for detection of HDAC1 by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC1 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (1,2). HDAC1 gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (3,4).
Background References:
  • Taunton J, Hassig CA, and Schreiber SL. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 1996; 272:408-11.
  • Cai RL, Yan-Neale Y, Cueto MA, et al. HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins. J. Biol. Chem. 2000; 275:27909-16.
  • Winter M, Moser MA, Meunier D, et al. Divergent roles of HDAC1 and HDAC2 in the regulation of epidermal development and tumorigenesis. EMBO J. 2013; 32:3176-91.
  • Turgeon N, Blais M, Gagn? JM, et al. HDAC1 and HDAC2 restrain the intestinal inflammatory response by regulating intestinal epithelial cell differentiation. PLoS One 2013; 8:e73785.
Buffer:
HDAC1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
HDAC1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human HDAC1.

The immunogen is located within the last 50 amino acids of HDAC1.
NCBI Gene ID #:
3065
NCBI Official Name:
histone deacetylase 1
NCBI Official Symbol:
HDAC1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 53 kDa

Observed: 54 kDa
Protein Accession #:
NP_004955
Protein GI Number:
13128860
Purification:
HDAC1 antibody is affinity chromatography purified via peptide column.
Research Area:
Cell Cycle,Stem Cell
SPECIFICITY:
HDAC1 antibody is human, mouse and rat reactive. HDAC1 antibody is predicted to not cross-react with other members of the HDAC family.
Swissprot #:
Q13547
User NOte:
Optimal dilutions for each application to be determined by the researcher.