HDAC2 Antibody
| Code | Size | Price |
|---|
| PSI-7899-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7899-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 293336691
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
HDAC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
HDAC2 Antibody: HD2, RPD3, YAF1, Histone deacetylase 2, HD2
Application Note:
HDAC2 antibody can be used for detection of HDAC2 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC2 was first identified as as a mammalian homolog to the yeast transcriptional regulator RPD3 (2). HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor (2,3). Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events (4).
Background References:
- Taunton J, Hassig CA, and Schreiber SL. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 1996; 272:408-11.
- Yang WM, Inouye C, Zeng Y, et al. Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3. Proc. Natl. Acad. Sci. USA 1996; 93:12845-50.
- Cress WD and Seto E. Histone deacetylases, transcriptional control, and cancer. J. Cell Phys. 2000; 184:1-16.
- Kramer OH. HDAC2: a critical factor in health and disease. Trends Pharmacol. Sci. 2009; 30:647-55.
Buffer:
HDAC2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (94%)
Immunogen:
HDAC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human HDAC2.
The immunogen is located within the last 50 amino acids of HDAC2.
The immunogen is located within the last 50 amino acids of HDAC2.
NCBI Gene ID #:
3066
NCBI Official Name:
histone deacetylase 2
NCBI Official Symbol:
HDAC2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 54 kDa
Observed: 52 kDa
Observed: 52 kDa
Protein Accession #:
NP_001518
Protein GI Number:
293336691
Purification:
HDAC2 antibody is affinity chromatography purified via peptide column.
Research Area:
Cell Cycle,Stem Cell
SPECIFICITY:
HDAC2 antibody is human, mouse and rat reactive. At least two isoforms of HDAC2 are known to exist; this antibody will detect both isoforms. HDAC2 antibody is predicted to not cross-react with other members of the HDAC family.
Swissprot #:
Q92769
User NOte:
Optimal dilutions for each application to be determined by the researcher.