USP25 Antibody
| Code | Size | Price |
|---|
| PSI-7765-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7765-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 50312666
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
USP25 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
USP25 Antibody: USP21, USP21, Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25
Application Note:
USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).
Background References:
- Valero R, Marfany G, Gonzalez-Angulo O, et al. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics 1999; 62:395-405.
- Valero R, Bayes M, Francisca Sanchez-Font M, et al. Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25. Genome Biol. 2001; 2: RESEARCH0043.
- Zhong B, Liu X, Wang X, et al. Negative regulation of IL-17-mediated signaling and inflammation by the ubiquitin-specific protease USP25. Nat. Immunol. 2012; 13:1110-7.
- Zhong B, Liu X, Wang X, et al. Ubiquitin-specific protease 25 regulates TLR4-dependent innate immune responses through deubiquitination of the adaptor protein TRAF3. Sci. Signal. 2013; 6:ra35.
Buffer:
USP25 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25.
The immunogen is located within amino acids 470 - 520 of USP25.
The immunogen is located within amino acids 470 - 520 of USP25.
NCBI Gene ID #:
29761
NCBI Official Name:
ubiquitin specific peptidase 25
NCBI Official Symbol:
USP25
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 116 kDa
Observed: 105 kDa
Observed: 105 kDa
Protein Accession #:
NP_037528
Protein GI Number:
50312666
Purification:
USP25 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.
Swissprot #:
Q9UHP3
User NOte:
Optimal dilutions for each application to be determined by the researcher.