OASL Antibody
| Code | Size | Price |
|---|
| PSI-7775-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7775-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 11321577
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
OASL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
OASL Antibody: OASLd, TRIP14, TRIP-14, p59OASL, p59 OASL, p59-OASL, Thyroid receptor-interacting protein 14, TR-interacting protein 14
Application Note:
OASL antibody can be used for detection of OASL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
OASL (2'-5'-oligoadenylate synthetase-like), also known as p59OASL or TRIP14 (thyroid receptor-interacting protein 14), is a 514 amino acid protein that exists as two alternatively spliced isoforms, designated p56 and p30, and contains two ubiquitin-like domains (1). It is widely expressed in a variety of tissues and interacts with the ligand binding domain of the thyroid receptor (TR) and is able to bind double-stranded RNA and DNA, possibly playing a role in RNA degradation and the overall inhibition of protein synthesis (2-3). Methyl CpG-binding protein 1 (MBD1), which functions as a transcriptional repressor, was identified as a strong p59 OASL interactor (4).
Background References:
- Rebouillat D, Marie I, and Hovanessian AG. Molecular cloning and characterization of two related and interferon-induced 56 kDa and 30 kDa proteins highly similar to 2'-5' oligoadenylate synthetase. Eur. J. Biochem. 1998; 257:319-30.
- Hartmann R, Olsen HS, Widder S, et al. p59OASL, a 2'-5' oligoadenylate synthetase like protein: a novel human gene related to the 2'-5' oligoadenylate synthetase family. Nucleic Acids Res. 1998; 26:4121-8.
- Hovnanian A, Rebouillat D, Levy ER, et al. The human 2',5'-oligoadenylate synthetaselike gene (OASL) encoding the interferon-induced 56 kDa protein maps to chromosome 12q24.2 in the proximity of the 2',5'-OAS locus. Genomics 1999; 56:362-3.
- Andersen JB, Strandbyg?rd DJ, Hartmann R, et al. Interaction between the 2'-5' oligoadenylate synthetase-like protein p59 OASL and the transcriptional repressor methyl CpG-binding protein 1. Eur. J. Biochem. 2004; 271:628-36.
Buffer:
OASL Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
OASL antibody was raised against a 17 amino acid peptide near the amino terminus of human OASL.
The immunogen is located within the first 50 amino acids of OASL.
The immunogen is located within the first 50 amino acids of OASL.
NCBI Gene ID #:
8638
NCBI Official Name:
2'-5'-oligoadenylate synthetase-like
NCBI Official Symbol:
OASL
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 57 kDa
Observed: 65 kDa
Observed: 65 kDa
Protein Accession #:
NP_003724
Protein GI Number:
11321577
Purification:
OASL antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
OASL antibody is human, mouse and rat reactive.
Swissprot #:
Q15646
User NOte:
Optimal dilutions for each application to be determined by the researcher.