DEPTOR Antibody

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ProSci
Product Code: PSI-7787
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7787-0.02mg0.02mg£150.00
Quantity:
PSI-7787-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 189571664
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
DEPTOR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of DEPTOR in mouse liver tissue lysate with DEPTOR antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of DEPTOR in mouse liver tissue with DEPTOR antibody at 5 μg/mL.
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Western blot analysis of DEPTOR in mouse liver tissue lysate with DEPTOR antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of DEPTOR in mouse liver tissue with DEPTOR antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
DEPTOR Antibody: DEP.6, DEPDC6, DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6
Application Note:
DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).
Background References:
  • Peterson TR, Laplante M, Thoreen CC, et al. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for survival. Cell 2009; 137:873-6.
  • Duan S, Skaar JR, Kuchay S, et al. mTOR generates an auto-amplification loop by triggering the bTrCP- and CK1a-dependent degradation of DEPTOR. Mol. Cell 2011; 44:317-24.
Buffer:
DEPTOR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR.

The immunogen is located within amino acids 220 - 270 of DEPTOR.
NCBI Gene ID #:
64798
NCBI Official Name:
DEP domain containing MTOR-interacting protein
NCBI Official Symbol:
DEPTOR
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 45 kDa

Observed: 42 kDa
Protein Accession #:
NP_073620
Protein GI Number:
189571664
Purification:
DEPTOR antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q8TB45
User NOte:
Optimal dilutions for each application to be determined by the researcher.