SKI2W Antibody
| Code | Size | Price |
|---|
| PSI-7987-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7987-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 67782311
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
SKI2W antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
G-protein coupled receptor-associated sorting protein 1, GASP, GASP-1, GASP1
Application Note:
GPRASP1 antibody can be used for detection of GPRASP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The G protein-coupled receptor associated sorting protein 1 (GPRASP1) is a member of the GPRASP family and is thought to modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors (1,2). GPRASP1 interacts with receptors such as the D2 dopamine receptor (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor (CNR1) and targets these proteins for degradation in lysosomes (2). Recently, it has been shown that increased GPRASP1 levels may act as a potential biomarker for breast cancer (3).
Background References:
- Simonin F, Karcher P, Boeuf JJ, et al. Identification of a novel family of G protein-coupled receptor associated proteins. J. Neurochem. 2004; 89:766-75.
- Moser E, Kargl J, Whistler JL, et al. G protein-coupled receptor-associated sorting protein 1 regulates the postendocytic sorting of seven-transmembrane-spanning G protein-coupled receptors. Pharmacology 2010; 86:22-9.
- Tuszynski GP, Rothman VL, Zheng X, et al. G-protein coupled receptor-associated sorting protein 1 (GASP-1), a potential biomarker in breast cancer. Exp. Mol. Pathol. 2011; 91:608-13.
Buffer:
GPRASP1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GPRASP1 antibody was raised against a 15 amino acid peptide near the amino terminus of human GPRASP1.
The immunogen is located within amino acids 260 - 310 of GPRASP1.
The immunogen is located within amino acids 260 - 310 of GPRASP1.
NCBI Gene ID #:
9737
NCBI Official Name:
G protein-coupled receptor associated sorting protein 1
NCBI Official Symbol:
GPRASP1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 153 kDa
Observed: 150 kDa
Observed: 150 kDa
Protein Accession #:
NP_001171656
Protein GI Number:
296080706
Purification:
GPRASP1 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
GPRASP1 antibody is human and mouse reactive. This antibody is predicted to not cross-react with GPRASP2.
Swissprot #:
Q5JY77
User NOte:
Optimal dilutions for each application to be determined by the researcher.