GJB2 Antibody

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ProSci
Product Code: PSI-8031
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8031-0.02mg0.02mg£150.00
Quantity:
PSI-8031-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 42558283
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
GJB2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of GJB2 in human colon tissue lysate with GJB2 antibody at (A) 1 and (B) 2 μg/ml.
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Western blot analysis of GJB2 in human colon tissue lysate with GJB2 antibody at (A) 1 and (B) 2 μg/ml.

Documents

Further Information

Additional Names:
Gap junction beta-2 protein, connexin 26, CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, HID, KID, NSRD1, PPK
Application Note:
GJB2 antibody can be used for detection of GJB2 by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
The Gap junction beta-2 protein (GJB2), also known as Connexin 26, is member of the gap junction protein family which form structures that were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells (1). Mutations in the GJB2 gene are thought to be responsible for as much as 35-45% of congenital sensorineural hearing loss in some populations (2). Other mutations in this gene have also been linked to a wide array of skin diseases (3).
Background References:
  • Zhou JZ and Jiang JX. Gap junctions and hemichannel-independent actions of connexins on cell and tissue functions ? An update. FEBS Lett. 2014; 588:1186-92.
  • Petit C, Levilliers J, and Hardelin JP. Molecular genetics of hearing loss. Annu. Rev. Genet. 2001; 35:589-646.
  • Gerido DA and White TW. Connexin disorders of the ear, skin, and lens. Biochim. Biophys. Acta. 2004; 1662:159-70.
Buffer:
GJB2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%), Mouse: (94%), Sheep: (88%), Bovine: (88%), Chicken: (82%)
Immunogen:
GJB2 antibody was raised against a 16 amino acid peptide near the center of human GJB2.

The immunogen is located within amino acids 80 - 130 of GJB2.
NCBI Gene ID #:
2706
NCBI Official Name:
gap junction protein, beta 2, 26kDa
NCBI Official Symbol:
GJB2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 25 kDa

Observed: 26 kDa
Protein Accession #:
NP_003995
Protein GI Number:
42558283
Purification:
GJB2 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
GJB2 antibody is human specific.
Swissprot #:
P29033
User NOte:
Optimal dilutions for each application to be determined by the researcher.