CRH Antibody
| Code | Size | Price |
|---|
| PSI-7797-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7797-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 4503041
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
CRH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
CRH Antibody: CRF, Corticoliberin, Corticotropin-releasing factor, CRF
Application Note:
CRH antibody can be used for detection of CRH by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Corticotropin-releasing hormone (CRH) is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress (1). In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta where it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturitio (2). Marked reduction in this protein has been observed in association with Alzheimer disease (3).
Background References:
- Maras PM and Baram TZ. Sculpting the hippocampus from within: stress, spines, and CRH. Trends Neurosci. 2012; 315-24.
- Vrachnis N, Malamas FM, Sifakis S, et al. Immune aspects and myometrial actions of progesterone and CRH in labor. Clin. Dev. Immunol. 2012; 937618.
- Leake A, Perry EK, Perry RH, et al. Neocortical concentrations of neuropeptides in senile dementia of the Alzheimer and Lewy body type: comparison with Parkinson's disease and severity correlations. Biol. Psychiatry 1991; 29:357-64.
Buffer:
CRH antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CRH antibody was raised against a 16 amino acid peptide near the amino terminus of human CRH.
The immunogen is located within amino acids 30 - 80 of CRH.
The immunogen is located within amino acids 30 - 80 of CRH.
NCBI Gene ID #:
1392
NCBI Official Name:
corticotropin releasing hormone
NCBI Official Symbol:
CRH
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 22 kDa
Observed: 29 kDa
Observed: 29 kDa
Protein Accession #:
NP_000747
Protein GI Number:
4503041
Purification:
CRH antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Neuroscience
SPECIFICITY:
CRH antibody is human specific.
Swissprot #:
P06850
User NOte:
Optimal dilutions for each application to be determined by the researcher.