WWC1 Antibody

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ProSci
Product Code: PSI-7991
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7991-0.02mg0.02mg£150.00
Quantity:
PSI-7991-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 242247251
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
WWC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of WWC1 in human brain tissue lysate with WWC1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of WWC1 in mouse brain tissue with WWC1 antibody at 5 μg/ml.
3 / 3
Immunofluorescence of WWC1 in mouse brain tissue with WWC1 antibody at 20 μg/ml.
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Western blot analysis of WWC1 in human brain tissue lysate with WWC1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of WWC1 in mouse brain tissue with WWC1 antibody at 5 μg/ml.
Immunofluorescence of WWC1 in mouse brain tissue with WWC1 antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
WW and C2 domain containing 1, HBEBP3, HBEBP36, KIBRA, MEMRYQTL
Application Note:
WWC1 antibody can be used for detection of WWC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
The WW and C2 domain containing 1 (WWC1) protein, also known as KIBRA, possesses two WW domains and an internal C2-like domain (1). WWC1 was originally identified as a memory performance-associated protein in humans (2) and has recently been shown to be a novel regulator of the Hippo pathway (3). WWC1 is phosphorylated by the mitotic kinases Aurora-A and ?B (4), and in turn activates the Aurora kinases and is required for precise chromosome alignment during mitosis (5).
Background References:
  • Kremerskothen J, Plaas C, Buther K, et al. Characterization of KIBRA, a novel WW domain-containing protein. Biochem. Biophys. Res. Commun. 2003; 300:862-7.
  • Papassotiropoulos A, Stephan DA, Huentelman MJ, et al. Common Kibra alleles are associated with human memory performance. Science 2006; 314:475-8.
  • Genevet A, Wehr MC, Brain R, et al. Kibra is a regulator of the Salvador/Warts/Hippo signaling network. Dev. Cell 2010; 18:309-16.
  • Xiao L, Chen Y, Ji M, et al. KIBRA protein phosphorylation is regulated by mitotic kinase aurora and protein phosphatase 1. J. Biol. Chem. 2011; 286:36304-15.
Buffer:
WWC1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
WWC1 antibody was raised against an 18 amino acid peptide near the amino terminus of human WWC1.

The immunogen is located within amino acids 60 - 110 of WWC1.
NCBI Gene ID #:
23286
NCBI Official Name:
WW and C2 domain containing 1
NCBI Official Symbol:
WWC1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 123 kDa

Observed: 110 kDa
Protein Accession #:
NP_001155133
Protein GI Number:
242247251
Purification:
WWC1 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Neuroscience
SPECIFICITY:
WWC1 antibody is human, mouse and rat reactive. At least three isoforms of WWC1 are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q8IX03
User NOte:
Optimal dilutions for each application to be determined by the researcher.