WAPL Antibody
| Code | Size | Price |
|---|
| PSI-7799-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7799-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 42734325
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
WAPL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
WAPL Antibody: FOE, WAPL, KIAA0261, FOE, Wings apart-like protein homolog, Friend of EBNA2 protein
Application Note:
WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
WAPL (wings apart-like), also known as WAPAL or FOE, is a 1,190 amino acid protein that contains one WAPL domain and may play an important role in cell growth (1). It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1) (1,2,4). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits (3,4). WAPL is a new regulator of the development and metastasis of cancerous tissue (4,5).
Background References:
- Kwiatkowski BA, Ragoczy T, Ehly J, et al. Identification and cloning of a novel chromatin-associated protein partner of Epstein-Barr nuclear protein 2. Exp. Cell Res. 2004; 300:223-33.
- Kueng S, Hegemann B, Peters BH, et al. Wapl controls the dynamic association of cohesion with chromatin. Cell 2006; 127:955-67.
- Gandhi R, Gillespie PJ, and Hirano T. Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Curr. Biol. 2006; 16:2406-17.
- Oikawa K, Ohbayashi T, Kiyono T, et al. Expression of a novel human gene, human wings apart-like (hWAPL), is associated with cervical carcinogenesis and tumor progression. Cancer Res. 2004; 64:3545-9.
Buffer:
WAPL antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
WAPL antibody was raised against an 18 amino acid peptide near the amino terminus of human WAPL.
The immunogen is located within amino acids 30 - 80 of WAPL.
The immunogen is located within amino acids 30 - 80 of WAPL.
NCBI Gene ID #:
23063
NCBI Official Name:
wings apart-like homolog (Drosophila)
NCBI Official Symbol:
WAPAL
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 131 kDa
Observed: 140 kDa
Observed: 140 kDa
Protein Accession #:
NP_055860
Protein GI Number:
42734325
Purification:
WAPL antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Stem Cell
SPECIFICITY:
WAPL antibody is human, mouse and rat reactive. At least four isoforms of WAPL are known to exist.
Swissprot #:
Q7Z5K2
User NOte:
Optimal dilutions for each application to be determined by the researcher.