TRIP12 Antibody
| Code | Size | Price |
|---|
| PSI-7953-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7953-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 10863903
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
TRIP12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
TRIP12 Antibody: ULF, TRIP-12, KIAA0045, ULF, E3 ubiquitin-protein ligase TRIP12, E3 ubiquitin-protein ligase for Arf
Application Note:
TRIP12 antibody can be used for detection of TRIP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunocytochemistry start at 2 μg/mL.For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples; Immunocytochemistry in human samples; and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples; Immunocytochemistry in human samples; and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1). TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2,3). TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4). TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5).
Background References:
- Lee JW, Choi HS, Gyuris J, et al. Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor. Mol. Endocrinol. 1995; 9:243?54.
- An CI, Ganio E, and Hagiwara N. Trip12, a HECT domain E3 ubiquitin ligase, targets Sox6 for proteasomal degradation and affects fiber type-specific gene expression in muscle cells. Skelet. Muscle 2013; 3:11.
- Poulsen EG, Steinhauer C, Lees M, et al. HUWE1 and TRIP12 collaborate in degradation of ubiquitin-fusion proteins and misframed ubiquitin. PLoS One. 2012; 7:e50548.
- Park Y, Yoon SK, and Yoon JB. The HECT domain of TRIP12 ubiquitinates substrates of the ubiquitin fusion degradation pathway. J. Biol. Chem. 2009; 284:1540-9.
Buffer:
TRIP12 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
TRIP12 antibody was raised against a 18 amino acid peptide near the carboxy terminus of human TRIP12.
The immunogen is located within amino acids 1930 - 1980 of TRIP12.
The immunogen is located within amino acids 1930 - 1980 of TRIP12.
NCBI Gene ID #:
9320
NCBI Official Name:
Thyroid hormone receptor interactor 12
NCBI Official Symbol:
TRIP12
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 219 kDa
Observed: 220 kDa
Observed: 220 kDa
Protein Accession #:
NP_004229
Protein GI Number:
10863903
Purification:
TRIP12 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Stem Cell
SPECIFICITY:
TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist.
Swissprot #:
Q14669
User NOte:
Optimal dilutions for each application to be determined by the researcher.