DOCK8 Antibody

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ProSci
Product Code: PSI-7847
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7847-0.02mg0.02mg£150.00
Quantity:
PSI-7847-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 238231392
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
DOCK8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of DOCK8 in EL4 cell lysate with DOCK8 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of DOCK8 in human spleen tissue with DOCK8 antibody at 5 μg/mL.
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Western blot analysis of DOCK8 in EL4 cell lysate with DOCK8 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of DOCK8 in human spleen tissue with DOCK8 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
DOCK8 Antibody: MRD2, ZIR8, HEL-205, Dedicator of cytokinesis protein 8
Application Note:
DOCK8 antibody can be used for detection of DOCK8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
The Dedicator of cytokinesis protein 8 (DOCK8) is a member of the DOCK180 family of guanine nucleotide exchange factors (1). DOCK8 plays an essential role in humoral immune responses and is important in the proper formation of the B cell immunological synapse (reviewed in 2). Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome (3).
Background References:
  • Ruusala A and Aspenstrom P. Isolation and characterisation of DOCK8, a member of the DOCK180-related regulators of cell morphology. FEBS Lett. 2004; 572:159-66.
  • Randall KL, Lambe T, Goodnow CC, et al. The essential role of DOCK8 in humoral immunity. Dis. Markers 2010; 29:141-50.
  • Engelhardt KR, McGhee S, Sinkler S, et al. Large deletions and point mutations involving the dedicator of cytokinesis 8 (DOCK8) in the autosomal recessive form of hyper-IgE syndrome. J. Allergy Clin. Immunol. 2009; 124:1289-302.
Buffer:
DOCK8 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DOCK8 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DOCK8.

The immunogen is located within the last 50 amino acids of DOCK8.
NCBI Gene ID #:
81704
NCBI Official Name:
dedicator of cytokinesis 8
NCBI Official Symbol:
DOCK8
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 166, 220, 231 kDa

Observed: 220, 230 kDa
Protein Accession #:
NP_982272
Protein GI Number:
238231392
Purification:
DOCK8 antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
DOCK8 antibody is human, mouse and rat reactive. Multiple isoforms of DOCK8 are known to exist.
Swissprot #:
Q8NF50
User NOte:
Optimal dilutions for each application to be determined by the researcher.