NOX1 Antibody
| Code | Size | Price |
|---|
| PSI-7921-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7921-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 148536873
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
NOX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
NOX1 Antibody: MOX1, NOH1, NOH-1, GP91-2, MOX1, NADPH oxidase 1, Mitogenic oxidase 1, NOX-1
Application Note:
NOX1 antibody can be used for detection of NOX1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress (1). NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox (1). Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv (2). NOH-1S is a voltage-gated proton channel that participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain (1-3). NOX1 have the potential to be effective treatments for a range of ischemic diseases (4).
Background References:
- Helmcke I, Heumuller S, Tikkanen R, et al. Identification of structural elements in Nox1 and Nox4 controlling localization and activity. Antioxid. Redox Signal. 2009; 11:1279-87.
- Piccoli C, D'Aprile A, Ripoli M, et al. Bone-marrow derived hematopoietic stem/progenitor cells express multiple isoforms of NADPH oxidase and produce constitutively reactive oxygen species. Biochem. Biophys. Res. Commun. 2007; 353:965-72.
- Lee JG, Lim EJ, Park DW, et al. A combination of Lox-1 and Nox1 regulates TLR9-mediated foam cell formation. Cell Signal. 2008; 20:2266-75.
- Stanic B, Katsuyama M, and Miller FJ Jr. An oxidized extracellular oxidation-reduction state increases Nox1 expression and proliferation in vascular smooth muscle cells via epidermal growth factor receptor activation. Arterioscler. Thromb. Vasc. Biol. 2010; 30:2234-41.
Buffer:
NOX1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (93%), Mouse: (85%)
Immunogen:
NOX1 antibody was raised against a 15 amino acid peptide near the center of human NOX1.
The immunogen is located within amino acids 210 - 260 of NOX1.
The immunogen is located within amino acids 210 - 260 of NOX1.
NCBI Gene ID #:
27035
NCBI Official Name:
NADPH oxidase 1
NCBI Official Symbol:
NOX1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 62 kDa
Observed: 64 kDa
Observed: 64 kDa
Protein Accession #:
NP_008983
Protein GI Number:
148536873
Purification:
NOX1 antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
NOX1 antibody is human specific.
Swissprot #:
Q9Y5S8
User NOte:
Optimal dilutions for each application to be determined by the researcher.