NOX4 Antibody
| Code | Size | Price |
|---|
| PSI-7927-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7927-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 8393843
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
NOX4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
NAPDH oxidase 4, NOX-4, RENOX, KOX, KOX-1
Application Note:
NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS) (2,3). Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active (4). The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism.
Background References:
- Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313.
- Cheng G, Cao Z, Xu X, et al. Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5. Gene 2001; 269:131-40.
- Montezano AC, Burger D, Ceravolo GS, et al. Novel Nox homologues in the vasculature: focusing on Nox4 and Nox5. Clin. Sci. 2011; 120:131-41.
- Martyn KD, Frederick LM, von Loehneysen K, et al. Functional analysis of Nox4 reveals unique characteristics compared to other NAPDH oxidases. Cell. Signal. 2006; 18:69-82.
Buffer:
NOX4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
NOX4 antibody was raised against a 14 amino acid peptide near the amino terminus of human NOX4.
The immunogen is located within amino acids 70 - 120 of NOX4.
The immunogen is located within amino acids 70 - 120 of NOX4.
NCBI Gene ID #:
50507
NCBI Official Name:
NADPH oxidase 4
NCBI Official Symbol:
NOX4
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 64 kDa
Observed: 68 kDa
Observed: 68 kDa
Protein Accession #:
NP_058627
Protein GI Number:
8393843
Purification:
NOX4 antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
NOX4 antibody is human, mouse, and rat reactive. At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins.
Swissprot #:
Q9NPH5
User NOte:
Optimal dilutions for each application to be determined by the researcher.