DAF Antibody

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ProSci
Product Code: PSI-7937
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7937-0.02mg0.02mg£150.00
Quantity:
PSI-7937-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 168693643
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
DAF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 μg/ml.
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Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 μg/ml.
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Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 μg/ml.
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Western blot analysis of DAF1 expression in HeLa cell lysate with DAF1 antibody at (A) 1 and (B) 2 μg/ml.
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Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 μg/ml.
Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 μg/ml.
Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 μg/ml.
Western blot analysis of DAF1 expression in HeLa cell lysate with DAF1 antibody at (A) 1 and (B) 2 μg/ml.

Documents

Further Information

Additional Names:
Decay-accelerating factor, CD55 molecule decay accelerating factor for complement (Cromer blood group), CD55, CR, TC, CROM
Application Note:
DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The decay-accelerating factor (DAF), also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1). DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2). Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3).
Background References:
  • Nicholson-Weller A, March JP, Rosen CE, et al. Surface membrane expression by human blood leukocytes and platelts of decay-accelerating factor, a regulatory protein of the complement system. Blood 1985; 65:1237-44.
  • Seya T and Atkinson JP. Functional properties of membrane cofactor protein of complement. Biochem. J. 1989; 64:581-8.
  • Storry JR, Reid ME, and Yazer MH. The Cromer blood group system: a review. Immunohematology 2010; 109-18.
Buffer:
DAF antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF.

The immunogen is located within amino acids 360 - 410 of DAF.
NCBI Gene ID #:
1604
NCBI Official Name:
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
NCBI Official Symbol:
CD55
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 48 kDa

Observed: 49 kDa
Protein Accession #:
NP_001108224
Protein GI Number:
168693643
Purification:
DAF antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.
Swissprot #:
P08174
User NOte:
Optimal dilutions for each application to be determined by the researcher.