BATF3 Antibody

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ProSci
Product Code: PSI-7939
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7939-0.02mg0.02mg£150.00
Quantity:
PSI-7939-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 8924246
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
BATF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of BATF3 in 293 cell lysate with BATF3 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunohistochemistry of BATF3 in human kidney tissue with BATF3 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of BATF3 in human kidney tissue with BATF3 Antibody at 20 μg/mL.
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Western blot analysis of BATF3 in 293 cell lysate with BATF3 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of BATF3 in human kidney tissue with BATF3 antibody at 5 μg/mL.
Immunofluorescence of BATF3 in human kidney tissue with BATF3 Antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Basic leucine zipper transcriptional factor ATF-like 3, SNFT, JDP1, JUNDM1
Application Note:
BATF3 body can be used for detection of BATF3 by Western blot at 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
BATF3, also known as SNFT, belongs to the basic leucine zipper transcriptional family and functions as a transcriptional repressor when heterodimerizing with Jun (1) and can repress the transcription of IL-2 and MMP-1 (1,2). BATF3 is required for the development of CD8alpha+ classical dendritic cells (DCs) and the related CD103+ DCs that cross-present antigens to CD8 T cells (3,4).
Background References:
  • Iacobelli M, Wachsman W, and McGuire KL. Repression of IL-2 promoter activity by the novel basic leucine zipper p21SNFT protein. J. Immunol. 2000; 154:860-8.
  • Bower KE, Fritz JM, and McGuire KL. Transcriptional repression of MMP-1 by p21SNFT and reduced invasiveness of hepatocarcinoma cells. Oncogene 2004; 23:8805-14.
  • Hildner K, Edelson BT, Purtha WE, et al. BATF3 deficiency reveals a critical role for CD8alpha+ dendritic cells in cytotoxic T cell immunity. Science 2008; 322:1097-1100.
  • Edelson BT, Peripheral CD103+ dendritic cells form a unified subset developmentally related to CD8alpha+ conventional dendritic cells. J. Exp. Med. 2010; 207:823-36.
Buffer:
BATF3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BATF3 antibody was raised against a 16 amino acid peptide near the amino terminus of human BATF3.

The immunogen is located within the first 50 amino acids of BATF3.
NCBI Gene ID #:
55509
NCBI Official Name:
basic leucine zipper transcription factor, ATF-like 3
NCBI Official Symbol:
BATF3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 14 kDa

Observed: 17 kDa
Protein Accession #:
NP_061134
Protein GI Number:
8924246
Purification:
BATF3 antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology,Stem Cell
SPECIFICITY:
BATF3 antibody is human, mouse and rat reactive. BATF3 antibody is predicted to have no cross-reactivity to other BATF family members.
Swissprot #:
Q9NR55
User NOte:
Optimal dilutions for each application to be determined by the researcher.