CACNA1H Antibody
| Code | Size | Price |
|---|
| PSI-8029-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8029-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 53832009
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
CACNA1H antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
Calcium channel voltage-dependent T type alpha 1H subunit, CACNA1HB, Cav3.2, ECA6, EIG6
Application Note:
CACNA1H antibody can be used for detection of CACNA1H by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
The calcium channel protein CACNA1H is a T-type member of the alpha-1 subunit family that is part of the voltage-dependent calcium channel complex which mediates the influx of calcium ions into the cell upon membrane polarization (1,2). CACNA1H is a subunit of Cav3.2 T-type calcium channel that is involved in neurological disorders such as epilepsy and pain (2,3). CACNA1H associates with the caveolin protein Cav-3 and it is thought that Cav-3 regulates the Protein Kinase A (PKA) modulation of CACNA1H-containing Cav3.2 T-type calcium channels (4).
Background References:
- Cribbs LL, Lee JH, Yang J, et al. Cloning and characterization of alpha1H from human heart, a member of the T-type CA2+ channel gene family. Circ. Res. 1998; 83:103-9.
- Sekiguchi F and Kawabata A. T-type calcium channels: functional regulation and implication in pain signaling. J. Pharmacol. Sci. 2013; 122:244-50.
- McGivern JG. Targeting N-type and T-type calcium channels for the treatment of pain. Drug Discov. Today 2006; 11:245-53.
- Markandeya YS, Fahey JM, Pluteanu F, et al. Caveolin-3 regulates protein kinase A modulation of the Cav3.2 (a1H) T-type Ca2+ channels. J. Biol. Chem. 2011; 286:2433-2444.
Buffer:
CACNA1H antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CACNA1H antibody was raised against a 16 amino acid peptide near the center of human CACNA1H.
The immunogen is located within amino acids 1010 - 1060 of CACNA1H.
The immunogen is located within amino acids 1010 - 1060 of CACNA1H.
NCBI Gene ID #:
8912
NCBI Official Name:
calcium channel, voltage-dependent, T type, alpha 1H subunit
NCBI Official Symbol:
CACNA1H
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 259 kDa
Observed: 260 kDa
Observed: 260 kDa
Protein Accession #:
NP_066921
Protein GI Number:
53832009
Purification:
CACNA1H antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
CACNA1H antibody is human, mouse and rat reactive. Multiple isoforms of CACNA1H are known to exist.
Swissprot #:
O95180
User NOte:
Optimal dilutions for each application to be determined by the researcher.