PARK2 Antibody
| Code | Size | Price |
|---|
| PSI-7903-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7903-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 169790969
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
PARK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
PARK2 Antibody: PDJ, PRKN, AR-JP, LPRS2, E3 ubiquitin-protein ligase parkin, Parkinson juvenile disease protein 2, Parkinson disease protein 2
Application Note:
PARK2 antibody can be used for detection of PARK2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Parkinson?s disease (PD) is a neurodegenerative disease whose symptoms include tremors, rigidity, bradykinesia, and postural instability (1). Mutations in the PARK2 gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease (2). The PARK2 protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation (3). Recent studies have suggested that PARK2 expression reduces the mitochondrial accumulation of the apoptosis protein Bax under basal conditions and directly ubiquitinates Bax, thereby promoting cell survival (4).
Background References:
- Dauer W and Przedborski S. Parkinson's disease: Mechanisms and models. Neuron 2003; 39:889-909.
- Kitada T, Asakawa S, Hattori N, et al. Mutations in the parkin gene cause autosomal recessive juvenile parkinsonism. Nature 1998; 392:605-8.
- Shimura H, Hattori N, Kubo Si, et al. Familial Parkinson disease gene product, parkin, is a ubiquitin-protein ligase. Nat. Genet. 2000; 25:302-5.
- Johnson BN, Berger AK, Cortese GP, et al. The ubiquitin E3 ligase parkin regulates the proapoptotic function of Bax. Proc. Natl. Acad. Sci. USA 2012; 109:6283-88.
Buffer:
PARK2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PARK2 antibody was raised against a 17 amino acid peptide near the amino terminus of human PARK2.
The immunogen is located within amino acids 110 - 160 of PARK2.
The immunogen is located within amino acids 110 - 160 of PARK2.
NCBI Gene ID #:
5071
NCBI Official Name:
parkin RBR E3 ubiquitin protein ligase
NCBI Official Symbol:
PARK2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 51 kDa
Observed: 55 kDa
Observed: 55 kDa
Protein Accession #:
NP_004553
Protein GI Number:
169790969
Purification:
PARK2 antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience,Apoptosis
SPECIFICITY:
PARK2 antibody is human specific.
Swissprot #:
O60260
User NOte:
Optimal dilutions for each application to be determined by the researcher.