SREBF1 Antibody
| Code | Size | Price |
|---|
| PSI-7853-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7853-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 52630419
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
SREBF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
SREBF1 Antibody: SREBP1, bHLHd1, SREBP-1c, BHLHD1, SREBP1, Sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, SREBP-1
Application Note:
SREBF1 antibody can be used for detection of SREBF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The sterol regulatory element binding transcription factor 1 (SREBF1) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1). The related protein SREBF2 also binds SRE1 and activates transcription in an additive fashion to SREBF1 (2). SREBF1 is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1 (3). The SREBF1 proteins are important in the regulation of genes involved in lipid metabolism, while SREBF2 has been more closely associated with cholesterol synthesis and accumulation (4).
Background References:
- Wang X, Briggs MR, Hua X, et al. Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter. II. Purification and characterization. J. Biol. Chem. 1993; 268:14497-504.
- Hua X, Yokoyama C, Wu J, et al. SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc. Natl. Acad. Sci. USA 1993; 90:11603-7.
- Wang X, Sato R, Brown MS, et al. SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis. Cell 1994; 77:53-62.
- Raghow R, Yellaturu C, Deng X, et al. SREBPs: the crossroads of physiological and pathological homeostasis. Endocrinol. Metab. 2008; 19:65-73.
Buffer:
SREBF1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SREBF1 antibody was raised against a 17 amino acid peptide near the center of human SREBF1.
The immunogen is located within amino acids 450 - 500 of SREBF1.
The immunogen is located within amino acids 450 - 500 of SREBF1.
NCBI Gene ID #:
6720
NCBI Official Name:
sterol regulatory element binding transcription factor 1
NCBI Official Symbol:
SREBF1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 129 kDa
Observed: 132 kDa
Observed: 132 kDa
Protein Accession #:
NP_001005291
Protein GI Number:
52630419
Purification:
SREBF1 antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity
SPECIFICITY:
SREBF1 antibody is human and mouse reactive. At least three isoforms of SREBF1 are known to exist. SREBF1 antibody is predicted not to cross-react with SREBF2.
Swissprot #:
P36956
User NOte:
Optimal dilutions for each application to be determined by the researcher.