SREBF2 Antibody
| Code | Size | Price |
|---|
| PSI-7855-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7855-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 27477113
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
SREBF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
SREBF2 Antibody: SREBP2, bHLHd2, SREBP-2, BHLHD2, SREBP2, Sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2
Application Note:
SREBF2 antibody can be used for detection of SREBF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The sterol regulatory element binding transcription factor 2 (SREBF2) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1,2). The related protein SREBF1 also binds SRE1 and activates transcription in an additive fashion to SREBF2 (2). SREBF2 has been more closely associated with cholesterol synthesis and accumulation, while the SREBF1 proteins are important in the regulation of genes involved in lipid metabolism (3).
Background References:
- Hua X, Yokoyama C, Wu J, et al. SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc. Natl. Acad. Sci. USA 1993; 90:11603-7.
- Wang X, Briggs MR, Hua X, et al. Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter. II. Purification and characterization. J. Biol. Chem. 1993; 268:14497-504.
- Raghow R, Yellaturu C, Deng X, et al. SREBPs: the crossroads of physiological and pathological homeostasis. Endocrinol. Metab. 2008; 19:65-73.
Buffer:
SREBF2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Immunogen:
SREBF2 antibody was raised against a 15 amino acid peptide near the center of human SREBF2.
The immunogen is located within amino acids 440 - 490 of SREBF2.
The immunogen is located within amino acids 440 - 490 of SREBF2.
NCBI Gene ID #:
6721
NCBI Official Name:
sterol regulatory element binding transcription factor 2
NCBI Official Symbol:
SREBF2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 126 kDa
Observed: 128 kDa
Observed: 128 kDa
Protein Accession #:
NP_004590
Protein GI Number:
27477113
Purification:
SREBF2 antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity
SPECIFICITY:
SREBF2 antibody is human and mouse reactive. At least three isoforms of SREBF2 are known to exist. SREBF2 antibody is predicted not to cross-react with SREBF1.
Swissprot #:
Q12772
User NOte:
Optimal dilutions for each application to be determined by the researcher.