SHBG Antibody

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ProSci
Product Code: PSI-7935
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7935-0.02mg0.02mg£150.00
Quantity:
PSI-7935-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 7382460
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
SHBG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of SHBG in human liver tissue lysate with SHBG antibody at (A) 1 and (B) 2 μg/ml.
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Immunofluorescence of SHBG in mouse liver tissue with SHBG antibody at 20 μg/ml.
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Western blot analysis of SHBG in human liver tissue lysate with SHBG antibody at (A) 1 and (B) 2 μg/ml.
Immunofluorescence of SHBG in mouse liver tissue with SHBG antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
Sex hormone-binding globulin, ABP, SBP, TEBG
Application Note:
SHBG antibody can be used for detection of SHBG by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
SHBG is a steroid binding protein that was first described as a plasma protein secreted by the liver and is thought to participate in the regulation of steroid responses. SHBG transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers (1). Low plasma SHBG levels are associated with obesity, abdominal adiposity, metabolic syndrome, and predict the development of type 2 diabetes (2,3). Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus (4).
Background References:
  • Siiteri PK, Murai JT, Hammond GL, et al. The serum transport of steroid hormones. Recent Prog. Horm. Res. 1982; 38:457-510.
  • Li C, Ford ES, Li B, et al. Association of testosterone and sex hormone-binding globulin with metabolic syndrome and insulin resistance in men. Diabetes Care 2010; 33:1618-24.
  • Ding EL, Song Y, Manson JE, et al. Sex hormone-binding globulin and risk of type 2 diabetes in women and men. N. Engl. J. Med. 2009; 361:1152-63.
  • Hacihanefioglu B, Aybey B, Hakan Ozon Y, et al. Association of anthropometric, androgenic and insulin-related features with polymorphisms in exon 8 of SHBG gene in women with polycystic ovary syndrome. Gynecol. Endocrinol. 2013; 29:361-4.
Buffer:
SHBG antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (75%)
Immunogen:
SHBG antibody was raised against a 16 amino acid peptide near the center of human SHBG.

The immunogen is located within amino acids 220 - 270 of SHBG.
NCBI Gene ID #:
6462
NCBI Official Name:
sex hormone-binding globulin
NCBI Official Symbol:
SHBG
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 25, 26, 31, 32, 38, 44 kDa

Observed: 35 kDa
Protein Accession #:
NP_001031
Protein GI Number:
7382460
Purification:
SHBG antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity
SPECIFICITY:
SHBG antibody is human, mouse, and rat reactive. At least three isoforms of SHBG are known to exist; this antibody will detect all three isoforms.
Swissprot #:
P04278
User NOte:
Optimal dilutions for each application to be determined by the researcher.