MFN1 Antibody
| Code | Size | Price |
|---|
| PSI-7861-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7861-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 45269137
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
MFN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
MFN1 Antibody: hfzo1, hfzo2, Mitofusin-1, Fzo homolog
Application Note:
MFN1 antibody can be used for detection of MFN1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
Mitofusin 1 (MFN1) and the related protein MFN2 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (1,2). MFN1 and MFN2 form homotypic and heterotypic complexes and coordinately regulate mitochondrial fusion and are essential for embryonic development (3). When ectopically expressed, MFN1 inhibits the apoptosis-associated amino-terminal conformation change in the apoptotic protein Bax but not its mitochondrial translocation, indicating that MFN1 is involved in the regulating the activation of Bax on the outer mitochondrial membrane (4).
Background References:
- Chen H, Detmer SA, Ewald AJ, et al. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 2003; 160:189-200.
- Ishihara N, Eura Y, and Mihara K. Mitofusin 1 and 2 play distinct roles in mitochondrial fusion reactions via GTPase activity. J. Cell Sci. 2004; 117:6535-46.
- Chen H, Detmer SA, Ewald AJ, et al. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 2003; 160:189-200.
- Ryu SW, Choi K, Park JH, et al. Mitofusin 1 inhibits an apoptosis-associated amino-terminal conformational change in Bax, but not its mitochondrial translocation, in a GTPase-dependent manner. Cancer Lett. 2012; 323:62-8.
Buffer:
MFN1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MFN1 antibody was raised against a 17 amino acid peptide near the amino terminus of human MFN1.
The immunogen is located within amino acids 20 - 70 of MFN1.
The immunogen is located within amino acids 20 - 70 of MFN1.
NCBI Gene ID #:
55669
NCBI Official Name:
mitofusin 1
NCBI Official Symbol:
MFN1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 82 kDa
Observed: 83 kDa
Observed: 83 kDa
Protein Accession #:
NP_284941
Protein GI Number:
45269137
Purification:
MFN1 antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity,Apoptosis
SPECIFICITY:
MFN1 antibody is human, mouse and rat reactive. MFN1 antibody is predicted to not cross-react with MFN2.
Swissprot #:
Q8IWA4
User NOte:
Optimal dilutions for each application to be determined by the researcher.