MFN2 Antibody
| Code | Size | Price |
|---|
| PSI-7863-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7863-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 7662004
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
MFN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
MFN2 Antibody: HSG, MARF, CMT2A, CPRP1, CMT2A2, KIAA0214, Mitofusin-2, Transmembrane GTPase MFN2
Application Note:
MFN2 antibody can be used for detection of MFN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Background:
Mitofusin 2 (MFN2) and the related protein MFN1 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (1,2). MFN2 is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane. MFN2 has two key domains: a coiled coil region that mediates MFN2 binding and a GTPase domain that likely plays a role in fusion (3,4). Both domains are essential for embryonic development and may play a role in the pathobiology of obesity. Overexpression of MFN2 causes mitochondrial dysfunction and cell death (5).
Background References:
- Chen H, Detmer SA, Ewald AJ, et al. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 2003; 160:189-200.
- Ishihara N, Eura Y, and Mihara K. Mitofusin 1 and 2 play distinct roles in mitochondrial fusion reactions via GTPase activity. J. Cell Sci. 2004; 117:6535-46.
- Bach D, Pich S, Soriano FX, et al. Mitofusin-2 determines mitochondrial network architecture and mitochondrial metabolism. A novel regulatory mechanism altered in obesity. J. Biol. Chem. 2003; 278:17190-7.
- Renaldo F, Amati-Bonneau P, Slama A, et al. MFN2, a new gene responsible for mitochondrial DNA depletion. Brain 2012; 135:e223, 1-4.
Buffer:
MFN2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MFN2 antibody was raised against a 17 amino acid peptide near the center of human MFN2.
The immunogen is located within amino acids 250 - 300 of MFN2.
The immunogen is located within amino acids 250 - 300 of MFN2.
NCBI Gene ID #:
9927
NCBI Official Name:
mitofusin 2
NCBI Official Symbol:
MFN2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 83 kDa
Observed: 90 kDa
Observed: 90 kDa
Protein Accession #:
NP_055689
Protein GI Number:
7662004
Purification:
MFN2 antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity,Apoptosis
SPECIFICITY:
MFN2 antibody is human, mouse and rat reactive. At least three isoforms of MFN2 are known to exist. MFN2 antibody is predicted to not cross-react with MFN1.
Swissprot #:
O95140
User NOte:
Optimal dilutions for each application to be determined by the researcher.