SMURF2 Antibody
| Code | Size | Price |
|---|
| PSI-7773-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7773-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 12232397
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
SMURF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
SMURF2 Antibody: E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, hSMURF2
Application Note:
SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3). Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3). SMURF2 associates constitutively with SMAD7 (4).
Background References:
- Huibregtse JM, Scheffner M, Beaudenon S, et al. A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. Proc. Natl. Acad. Sci. USA 1995; 92:2563-7.
- Hwang YS, Lee HS, Kamata T, et al. The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1. Genes Dev. 2013; 27:491-503.
- Lin X, Liang M, and Feng XH. Smurf2 is a ubiquitin E3 ligase mediating proteasomedependent degradation of Smad2 in transforming growth factor-beta signaling. J. Biol. Chem. 2000; 275:36818-22.
- Kavsak P, Rasmussen RK, Causing CG, et al. Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF-beta receptor for degradation. Mol. Cell 2000; 6:1365-75.
Buffer:
SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2.
The immunogen is located within amino acids 140 - 190 of SMURF2.
The immunogen is located within amino acids 140 - 190 of SMURF2.
NCBI Gene ID #:
64750
NCBI Official Name:
SMAD specific E3 ubiquitin protein ligase 2
NCBI Official Symbol:
SMURF2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 82 kDa
Observed: 85 kDa
Observed: 85 kDa
Protein Accession #:
NP_073576
Protein GI Number:
12232397
Purification:
SMURF2 antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.
Swissprot #:
Q9HAU4
User NOte:
Optimal dilutions for each application to be determined by the researcher.