CASR Antibody
| Code | Size | Price |
|---|
| PSI-7785-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7785-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 296010811
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
CASR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
CASR Antibody: CAR, FHH, FIH, HHC, EIG8, HHC1, NSHPT, PCAR1, GPRC2A, HYPOC1, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, CaSR
Application Note:
CASR antibody can be used for detection of CASR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The calcium-sensing receptor protein (CASR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (1). It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis (1). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (1,2). Recent evidence suggests that activated CASR contributes to the cytokine secretion through the partial MAPK and NF-kappaB pathways in T cells (3).
Background References:
- Pollak MR, Brown EM, Chou YH, et al. Mutations in the human Ca(2+)-sensing receptor gene cause familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism. Cell 1993; 75:1297-303.
- Jakobsen SF, Rolighed L, Nissen PH, et al. Muscle function and quality of life are not impaired in familial hypocalciuric hypercalcemia: a cross-sectional study on physiological effects of inactivating variants in the calcium-sensing receptor gene (CASR). Eur. J. Endocrinol. 2013; 169:349-57.
- Li T, Sun M, Yin X, et al. Expression of the calcium sensing receptor in human peripheral blood T lymphocyte and its contribution to cytokine secretion through MAPKs or NF-kB pathways. Mol. Immunol. 2013; 53:414-20.
Buffer:
CASR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
CASR antibody was raised against a 15 amino acid peptide near the amino terminus of human CASR.
The immunogen is located within the first 50 amino acids of CASR.
The immunogen is located within the first 50 amino acids of CASR.
NCBI Gene ID #:
846
NCBI Official Name:
calcium-sensing receptor
NCBI Official Symbol:
CASR
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 101, 120 kDa
Observed: 115, 125 kDa
Observed: 115, 125 kDa
Protein Accession #:
NP_001171536
Protein GI Number:
296010811
Purification:
CASR antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
CASR antibody is human, mouse and rat reactive. At least two isoforms of CASR are known to exist.
Swissprot #:
P41180
User NOte:
Optimal dilutions for each application to be determined by the researcher.