PDCL Antibody

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ProSci
Product Code: PSI-7871
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7871-0.02mg0.02mg£150.00
Quantity:
PSI-7871-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 88758594
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
PDCL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of PDCL in K562 cell lysate with PDCL antibody at 1 μg/ml.
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Western blot analysis of PDCL in K562 cell lysate with PDCL antibody at 1 μg/ml.

Documents

Further Information

Additional Names:
PDCL Antibody: PhLP, PHLOP1, PhLP1, Phosducin-like protein, PHLP
Application Note:
PDCL antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1,2). PDCL is widely expressed in normal human tissues and may participate directly in the regulation of calcium-evoked exocytosis in adrenal medullary chromaffin cells (3). It is an ethanol-responsive modulator of heterotrimeric G proteins (4).
Background References:
  • Miles MF, Barhite S, Sganga M, et al. Phosducin-like protein: an ethanol-responsive potential modulator of guanine nucleotide-binding protein function. Proc. Natl. Acad. Sci. USA 1993; 90:10831-5.
  • Ruiz-Gomez A, Humrich J, Murga C, et al. Phosphorylation of phosducin and phosducinlike protein by G protein-coupled receptor kinase 2. J. Biol. Chem. 2000; 275:29724-30.
  • Gensse M, Vitale N, Chasserot-Golaz S, et al. Regulation of exocytosis in chromaffin cells by phosducin-like protein, a protein interacting with G protein betagamma-subunits. FEBS Lett. 2000; 480:184-8.
  • Barhite S, Thibault C, Miles MF, et al. Phosducin-like protein (PhLP), a regulator of G beta gamma function, interacts with the proteasomal protein SUG1. Biochim. Biophys. Acta 1998; 1402: 95-101.
Buffer:
PDCL antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
PDCL antibody was raised against a 16 amino acid peptide near the amino terminus of human PDCL.

The immunogen is located within the first 50 amino acids of PDCL.
NCBI Gene ID #:
5082
NCBI Official Name:
phosducin-like
NCBI Official Symbol:
PDCL
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 33 kDa

Observed: 35 kDa
Protein Accession #:
NP_005379
Protein GI Number:
88758594
Purification:
PDCL antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
PDCL antibody is human, mouse and rat reactive. At least two isoforms of PDCL are known to exist. PDCL antibody is predicted to not cross-react with other members of the PDCL protein family.
Swissprot #:
Q13371
User NOte:
Optimal dilutions for each application to be determined by the researcher.