PDCL3 Antibody
| Code | Size | Price |
|---|
| PSI-7889-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7889-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 13129044
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
PDCL3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
PDCL3 Antibody: VIAF, PHLP3, VIAF1, HTPHLP, PHLP2A, PhLP2A, Phosducin-like protein 3, VIAF-1
Application Note:
PDCL3 antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1,2). PDCL3 is highly homologous to PDCL and shares an N-terminal helix domain and a C-terminal thioredoxin-fold (Trx-fold) domain (3). Along with the related protein PDCL2, PDCL3 interacts with the chaperonin CCT and modulates CCT-mediated actin and tubulin folding (4). Modulation of PDCL3 levels by MAPK phosphorylation and RhoA-dependent changes also promote cytoskeletal remodeling (5).
Background References:
- Miles MF, Barhite S, Sganga M, et al. Phosducin-like protein: an ethanol-responsive potential modulator of guanine nucleotide-binding protein function. Proc. Natl. Acad. Sci. USA 1993; 90:10831-5.
- Ruiz-Gomez A, Humrich J, Murga C, et al. Phosphorylation of phosducin and phosducinlike protein by G protein-coupled receptor kinase 2. J. Biol. Chem. 2000; 275:29724-30.
- Lou X, Bao R, Zhou CZ, et al. Structure of the thioredoxin-fold domain of human phosducin-like protein 2. Acta Crystallographica 2009; F65:67-70.
- Stirling PC, Srayko M, Takhar KS, et al. Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeltal protein function and cell cycle progrssion. Mol. Biol. Cell 2007; 18:2336-45.
Buffer:
PDCL3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PDCL3 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human PDCL3.
The immunogen is located within the last 50 amino acids of PDCL3.
The immunogen is located within the last 50 amino acids of PDCL3.
NCBI Gene ID #:
79031
NCBI Official Name:
phosducin-like 3
NCBI Official Symbol:
PDCL3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 26 kDa
Observed: 25 kDa
Observed: 25 kDa
Protein Accession #:
NP_076970
Protein GI Number:
13129044
Purification:
PDCL3 antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
PDCL3 antibody is human, mouse and rat reactive. PDCL3 antibody is predicted to not cross-react with other members of the PDCL protein family.
Swissprot #:
Q9H2J4
User NOte:
Optimal dilutions for each application to be determined by the researcher.