RHOG Antibody
| Code | Size | Price |
|---|
| PSI-7981-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7981-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 46249393
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
RHOG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
Ras homolog family member G, Rho-related GTP-binding protein, ARHG
Application Note:
RHOG antibody can be used for detection of RHOG by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
The ras homolog family member G (RHOG), also known as rho-related GTP-binding protein, is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades (1). RHOG controls a pathway that requires the microtubule network and activates Rac1 and Cdc42Hs independently of their growth factor signaling pathway (2). RHOG is also involved in the transcriptional regulation of interferon-gamma and nuclear factor of activated T cells (NFAT) and the regulation of the actin skeleton in lymphocytes (3).
Background References:
- Vincent S, Janteur P, and Fort P. Growth-regulated of rhoG, a new member of the ras homolog family. Mol. Cell Biol. 1992; 12:3138-48.
- Gauthier-Rouviere C, Vignal E, Meriane M, et al. RhoG GTPase controls a pathway that independently activates Rac1 and Cdc42Hs. Mol. Biol. Cell 1998; 9:1379-94.
- Vigorito E, Billadeu DD, Savoy D, et al. RhoG regulates gene expression and the actin skeleton in lymphocytes. Oncogene 2003; 22:330-42.
Buffer:
RHOG antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (87%), Guinea pig: (87%), Chicken: (87%), Pig: (71%)
Immunogen:
RHOG antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOG.
The immunogen is located within amino acids 40 - 90 of RHOG.
The immunogen is located within amino acids 40 - 90 of RHOG.
NCBI Gene ID #:
391
NCBI Official Name:
ras homolog family member G
NCBI Official Symbol:
RHOG
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 21 kDa
Observed: 23 kDa
Observed: 23 kDa
Protein Accession #:
NP_001656
Protein GI Number:
46249393
Purification:
RHOG antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
RHOG antibody is human, mouse and rat reactive.
Swissprot #:
P84095
User NOte:
Optimal dilutions for each application to be determined by the researcher.