P2RX7 Antibody
| Code | Size | Price |
|---|
| PSI-8015-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8015-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 300068987
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
P2RX7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
P2X purinoceptor 7, P2X7, purinergic receptor P2X ligand-gated ion channel 7
Application Note:
P2RX7 antibody can be used for detection of P2RX7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
The purinergic receptor P2X ligand-gated ion channel 7 (P2RX7) belongs to the family of purinoceptors for ATP (1). This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules (1,2). Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression (2). Recent studies have suggested that P2RX7 may play a key role in immune-mediated diseases such as rheumatoid arthritis (3) as well as neuropsychiatric disorders (4).
Background References:
- Surprenant A, Rassendren F, Kawashima E, et al. The cytosolic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7). Science 1996; 272:735-8.
- North RA. Molecular physiology of P2X receptors. Physiol. Rev. 2002; 82:1013-67.
- Labasi JM, Petrushova N, Donovan C, et al. Absence of the P2X7 receptor alters leukocyte function and attenuates an inflammatory response. J. Immunol. 168:6436-45.
- Basso AM, Bratcher NA, Harris RR, et al. Behavioral profile of P2X7 receptor knockout mice in animal models of depression and anxiety; relevance for neuropsychiatric disorders. Behav. Brain Res. 2009; 198:83-90.
Buffer:
P2RX7 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (81%)
Immunogen:
P2RX7 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human P2RX7.
The immunogen is located within the last 50 amino acids of P2RX7.
The immunogen is located within the last 50 amino acids of P2RX7.
NCBI Gene ID #:
5027
NCBI Official Name:
purinergic receptor P2X, ligand-gated ion channel, 7
NCBI Official Symbol:
P2RX7
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 34, 47, 65 kDa
Observed: 54 kDa
Observed: 54 kDa
Protein Accession #:
NP_002553
Protein GI Number:
300068987
Purification:
P2RX7 antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Immunology
SPECIFICITY:
P2RX7 antibody is human, mouse, and rat reactive. Multiple isoforms of P2RX7 are known to exist.
Swissprot #:
Q99572
User NOte:
Optimal dilutions for each application to be determined by the researcher.