BAMBI Antibody
| Code | Size | Price |
|---|
| PSI-7931-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7931-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 6912534
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
BAMBI antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
BMP and activin membrane-bound inhibitor homolog
Application Note:
BAMBI antibody can be used for detection of BAMBI by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
BAMBI (BMP and activin membrane-bound inhibitor homolog, NMA) is a membrane spanning glycoprotein that acts as a negative regulator of TGF-beta signaling during development (1). BAMBI transcription regulation is under the influence of beta-catenin, BMP, smad3 and smad4 (2,3). BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues (4). BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines (5).
Background References:
- Onichtchouk D, Chen YG, Dosch R, et al. Silencing of TGF-beta signalling by the pseudoreceptor BAMBI. Nature 1999; 401:480-5.
- Yan X, Lin Z, Chen F, et al. Human BAMBI cooperates with Smad7 to inhibit transforming growth factor-beta signaling. J Biol. Chem. 2009; 284:30097-104.
- Grotewold L, Plum M, Dildrop R, et al. Bambi is coexpressed with Bmp-4 during mouse embryogenesis. Mech. Dev. 2001; 100:327-30.
- Fritzmann J, Morkel M, Besser D, et al. A colorectal cancer expression profile that includes transforming growth factor beta inhibitor BAMBI predicts metastatic potential. Gastroenterology 2009; 137:165-75.
Buffer:
BAMBI antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Sheep: (88%)
Immunogen:
BAMBI antibody was raised against a 17 amino acid peptide near the center of human BAMBI.
The immunogen is located within amino acids 100 - 150 of BAMBI.
The immunogen is located within amino acids 100 - 150 of BAMBI.
NCBI Gene ID #:
25805
NCBI Official Name:
BMP and activin membrane-bound inhibitor
NCBI Official Symbol:
BAMBI
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 29 kDa
Observed: 26 kDa
Observed: 26 kDa
Protein Accession #:
NP_036474
Protein GI Number:
6912534
Purification:
BAMBI antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Stem Cell
SPECIFICITY:
BAMBI antibody is human, mouse and rat reactive.
Swissprot #:
Q13145
User NOte:
Optimal dilutions for each application to be determined by the researcher.