ATMIN Antibody
| Code | Size | Price |
|---|
| PSI-7777-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7777-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 54792092
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
ATMIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
ATMIN Antibody: ASCIZ, ZNF822, KIAA0431, ATM interactor, Zinc finger protein 822, ASCIZ
Application Note:
ATMIN antibody can be used for detection of ATMIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The ATM/ATR-substrate CHK2-interacting zinc finger protein (ATMIN), also known as ASCIZ, forms DNA damage-induced nuclear foci that contain the DNA repair protein Rad51 (1). ATMIN is also thought to be involved in embryonic development, as an absence of ATMIN causes late-embryonic lethality in mice with a range of organ development defects (2). It also activates the transcription DYNLL1, a light chain of the dynein motor complex and sequence-specific regulator of protein dimerization of numerous targets. DYNLL1 can bind to and inhibit the transcription activation domain of ATMIN, forming a simple dynamic feedback loop for DYNLL1 expression (3).
Background References:
- McNees CJ, Conlan LA, Tenis N, et al. ASCIZ regulates lesion-specific Rad51 focus formation and apoptosis after methylating DNA damage. EMBO J. 2005; 24:2447-57.
- Jurado S, Smyth I, van Denderen B, et al. Dual functions of ASCIZ in the DNA base damage response and pulmonary organogenesis. PLoS Genet. 2010; 6:e1001170.
- Jurado S, Conlan LA, Baker EK, et al. ATM substrate Chk2-interating Zn2+ finger (ASCIZ) is a bi-functional transcriptional activator and feedback sensor in the regulation of Dynein Light Chain (DYNLL1) expression. J. Biol. Chem. 2012; 287:3156-64.
Buffer:
ATMIN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ATMIN antibody was raised against a 19 amino acid peptide near the amino terminus of human ATMIN.
The immunogen is located within amino acids 200 - 250 of ATMIN.
The immunogen is located within amino acids 200 - 250 of ATMIN.
NCBI Gene ID #:
23300
NCBI Official Name:
ATM interactor
NCBI Official Symbol:
ATMIN
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 73, 79, 91 kDa
Observed: 72 kDa
Observed: 72 kDa
Protein Accession #:
NP_056066
Protein GI Number:
54792092
Purification:
ATMIN antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
ATMIN antibody is human, mouse and rat reactive. At least three isoforms of ATMIN are known to exist.
Swissprot #:
O43313
User NOte:
Optimal dilutions for each application to be determined by the researcher.